农杆菌介导大豆未成熟子叶的遗传转化  

GENETIC TRANSFORMATION OF IMMATURE COTYLEDON VIA AGROBACTERIUM TUMEFACIENS IN SOYBEAN

作　　者：王萍[1] 王罡[1] 季静[1] 周智明[1] 郭威[1] 拉巴[1] 吴颖[1] 

机构地区：[1]解放军军需大学植物基因工程研究中心,长春130062

出　　处：《大豆科学》2004年第2期86-90,共5页Soybean Science

基　　金：国家植物转基因中试及产业化基地专项基金 (项目编号J99-B -0 0 1)

摘　　要：用农杆菌LBA4 40 4 (pGBI1 2 1S4ABC)转化 5个大豆品种的未成熟子叶 ,经卡那霉素筛选得到抗性植株 ,进一步用PCR检测 ,获得 1 0株PCR阳性植株。同时发现 ,不同大豆品种农杆菌转化的效率是不同的 ,吉林 4 3较易于转化 ;共培养时间是影响转化效率的主要因子。Immature cotyledon of 5 different soybean varieties were transformed by Agrobacterium tumefaciens LBA4404(pGBI121S4ABC) and resistant plants to kanamycin were obtained. 10 egenerated plants gave positive PCR reaction. Transformation efficiency was different among 5 varieties. Jilin 43 was easy for transforming by Agrobacterium tumefaciens. The time of co-cultivation is one of the most important factors for transformation efficiency.

关 键 词：农杆菌 大豆 子叶 遗传转化 品种 PCR检测 

分 类 号：S565.1[农业科学—作物学]