重组病毒趋化因子vMIP对脂多糖刺激的细胞免疫功能的影响  

Effects of recombinant viral chemokine vMIP-II on cellular immunity stimulated by LPS

作　　者：杨清玲[1] 孙晗笑[1] 

机构地区：[1]暨南大学基因组研究所,广东广州510632

出　　处：《中国病理生理杂志》2004年第5期788-791,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No .30 170 881);广东省重点科技攻关项目 (2 0 0 2A10 90 2 11);广州市重大科技计划项目 (2 0 0 2Z1-E4 0 2 1)

摘　　要：目的 :了解重组病毒趋化因子vMIP作用前后及内毒素刺激后细胞内细胞因子IL - 4和IFN -γ水平的变化 ,探讨vMIP对免疫功能的影响。方法 :通过放射性配体 -受体结合实验、ELISA法及流式CD4 + T细胞的细胞内染色法检测外周血单个核细胞的培养上清IL - 12水平及细胞内细胞因子IFN -γ及IL - 4分泌水平的变化。结果 :通过竞争结合细胞膜表面趋化因子受体 ,vMIP -II可减缓高浓度LPS引起的爆发式细胞因子IL - 12、IFN -γ产生 ;vMIP可促进IL - 12、IFN -γ和IL - 4分泌。结论 :病毒趋化因子vMIP -II可调节CD4 + T细胞分泌IFN -γ和IL -4 ,从而下调过激的炎症反应 。AIM: To investigate the produce of intracellular cytokine following short-term in vitro stimulation with vMIP and LPS, and discuss the effect of vMIP to cellular immunity. METHODS: The methods of Cross-linking of radioactivity, ELISA and four-colors flow cytometer were used to test the level of the secretion of chemokine IL-12 and intracellular cytokine IFN-γ and IL-4. RESULTS: After treated the PBMCs with vMIP-II, the levels of secretion of IL-12, IFN-γ and IL-4 were reduced in the present of LPS by competitively combining chemokine receptor; vMIP promoted CD4+T cell to secrete IL-12, IFN-γ and IL-4. CONCLUSION: vMIP-II can protect systemic response of immunity and reduce extremely inflammation by down-regulating proinflammation.

关 键 词：巨噬细胞炎性蛋白质类 脂多糖类 白细胞介素12 流式细胞术 

分 类 号：R392.11[医药卫生—免疫学]