柯萨奇B_4病毒诱导的实验性糖尿病小鼠血清一氧化氮浓度的变化  

Changes of serum nitric oxide in CB_4V-induced insulin-dependent diabetic mice

作　　者：黄红兰[1] 易世红[1] 李凡[1] 

机构地区：[1]吉林大学基础医学院病原生物学教研室,吉林长春130021

出　　处：《中国病理生理杂志》2004年第5期855-857,共3页Chinese Journal of Pathophysiology

摘　　要：目的 :观察CB4V诱导的实验性糖尿病小鼠血清NO浓度的变化以及在不同时期产生的IL - 1水平。方法 :建立CB4V诱导的实验性糖尿病小鼠模型 ,分别在病毒感染后 72h、1周、3周、6周、8周 ,用硝酸还原酶法测定血清NO-2 /NO-3 含量 ,同时测定经LPS诱导的巨噬细胞产生的IL - 1水平。结果 :(1)血清NO-2 /NO-3 浓度变化 :对照组各时期血清NO-2 /NO-3 浓度无显著差异 ,模型组小鼠血清NO-2 /NO-3 浓度在CB4V感染 72h后显著增高 (P <0 0 1) ,在感染后 1周达到高峰 ,6 - 8周时接近正常水平。 (2 )IL - 1水平的测定 :在CB4V感染后 72h ,IL - 1水平明显高于对照组 (P <0 0 1) ,1周达到高峰 (P <0 0 1) ,6周接近正常水平。在各时期NO-2 /NO-3 与IL - 1均呈高度正相关。结论 :NO可能参与CB4V诱导的IDDM发生发展过程。AIM: To observe the changes of serum nitric oxide and the production level of IL-1 in different period of coxsackievirus B 4 (CB 4V)-induced insulin-dependent diabetic mice.METHODS: The insulin-dependent diabetes mellitus (IDDM) animal model induced by CB 4V infection was established. Serum nitric oxide level was estimated by nitrate reductase method after infection 72 h,1 week, 3 weeks,6 weeks,8 weeks, respectively. At the same time, level of IL-1 produced by peritoneal Mф was measued.RESULTS: (1) Changes of serum nitric oxide: serum nitric oxide level in control group remained normal level. The serum nitric oxide level in diabetic group increased significantly at 72 h after infection(P<0.01), and reached top at 1 weeks after infection, then decreased to normal level in 6 and 8 weeks (P>0.05). (2) IL-1 activities: IL-1 activities were increased obviously from 72 h to 3 weeks after virus infection, but decreased to normal level after 6 weeks.CONCLUSION: Nitric oxide may be one of the important factors in the development of CB 4V-induced IDDM.

关 键 词：糖尿病 一氧化氮 白细胞介素-1 Β细胞 

分 类 号：R587.1[医药卫生—内分泌]