人免疫缺陷病毒1型(HIV-1)gag基因疫苗的免疫原性  

Study on the immunogenicity of HIV-1 gag vaccine

作　　者：江文正[1] 金宁一[1] 李子健[2] 张立树[1] 

机构地区：[1]解放军军需大学全军基因工程重点实验室,吉林长春130062 [2]吉林大学第一医院,吉林长春130023

出　　处：《细胞与分子免疫学杂志》2004年第3期272-273,共2页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家杰出青年科学基金资助 (No.3982 51 1 9);国家高技术研究发展计划 (863)资助项目(No.2 0 0 1AA2 1 50 31 )

摘　　要：目的 :检测人免疫缺陷病毒 1型 (HIV 1)gag基因疫苗的免疫原性。方法 :分别以ELISA、荧光抗体染色和乳酸脱氢酶释放法 ,检测免疫小鼠血清抗体滴度、脾T细胞亚群的数量和淋巴细胞杀伤效应。结果 :血清抗体滴度、脾T细胞亚群的数量及淋巴细胞杀伤效应 ,重组质粒pVAXGAG免疫组与空载体pVAX1对照组相比较差异显著(分别为P <0 .0 5和P <0 .0 1)。结论 :HIV 1DNA疫苗质粒pVAXGAG在BALB/c小鼠中不仅可诱导特异性体液免疫 ,而且可诱导特异性细胞免疫。AIM: To detect the immunogenicity of HIV 1 gag DNA vaccine. METHODS: The serum antibody titers of the immunized mice were detected by ELISA. The numbers of spleen’s CD4 + and CD8 + T cells were analyzed by the fluorescent antibody staining and the cytotoxic activity was determined by lactate dehydrogenase (LDH) release assay. RESULTS: The titers of the serum antibodies and the numbers and cytotoxic activity of spleen’s T lymphocyte subsets had significant differences ( P <0.05 and P <0.01, respectively) between pVAXGAG and pVAX1 vaccinated groups. CONCLUSION: HIV 1 DNA vaccine plasmid pVAXGAG could elicit both the specific humoral and celluar immunities in BALB/c mice.

关 键 词：人免疫缺陷病毒1型 GAG 核酸疫苗 免疫原性 

分 类 号：R373.9[医药卫生—病原生物学]