葡萄球菌肠毒素B突变体(K172E)的制备和抗肿瘤活性分析  

Preparation and the anti-tumor activity of mutant Staphylcoccal enterotoxin B

作　　者：邓小红[1] 郑玉玲[2] 郁枫[1] 宁保安[2] 姜永强[2] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]军事医学科学院微生物流行病研究所,北京100071

出　　处：《细胞与分子免疫学杂志》2004年第3期360-362,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目 (No.30 1 71 0 91 );北京市自然科学基金资助项目 (No .70 2 2 0 31 )

摘　　要：目的 :制备葡萄球菌B型肠毒素 (SEB)突变体 ,观察其抗肿瘤活性变化。方法 :将诱导表达的SEB突变体重组蛋白包涵体进行变性复性和分离纯化 ,并对其肿瘤抑制活性与野生型SEB进行比较。观察突变体蛋白的抑瘤效果。结果 :建立了可行的包涵体复性条件和纯化方法 ,获得的突变体蛋白具有明显的抗肿瘤作用 ,其抑瘤活性比野生型SEB至少高 10倍。结论 :获得的突变体SEB K172E的抗肿瘤效果经体外实验证实优于野生型SEB 。AIM: To prepare mutant Staphylococcal enterotoxin B(SEB) and observe its anti tumor activity. METHODS: The expressed mutant SEB K172E in inclusion body was denatured and renatured, and then isolated and purified. The anti tumor activity of the mutant SEB K172E was compared with wild type SEB. RESULTS: Renaturation and purification method of the mutant SEB K172E was developed. The anti tumor activity of acquired mutant protein was ten times higher than that of wild type SEB. CONCLUSION: The anti tumor activity of the mutant SEB K172E is much higher than that of wild type SEB, suggesting that the mutant SEB K172E may become a potential anti tumor drug.

关 键 词：葡萄球菌肠毒素B 突变体 复性 抗肿瘤活性 

分 类 号：R392.11[医药卫生—免疫学]