双黄益骨方对去卵巢糖尿病骨质疏松大鼠骨代谢的影响  

Effect of Shuanghuangyigu Recipe(双黄益骨片) on Bone Metabolism inOvariectomized Diabetes Rats

作　　者：李赛美[1] 陈长青[1] 熊莉华[1] 杨艳红[2] 邓长青[1] 朱艳芳[1] 冯鑫[1] 何敏[1] 

机构地区：[1]广州中医药大学附属第一医院,广东广州510405 [2]广东省人民医院,广东广州510080

出　　处：《中医药学刊》2004年第5期778-779,共2页Study Journal of Traditional Chinese Medicine

基　　金：广东省中医药管理局课题 ( 2 0 0 0 -2 0 0 2年 ) (B10 0 0 48)

摘　　要：目的 :采用卵巢摘除术 +链脲佐菌素复合方法建立绝经后糖尿病骨质疏松症大鼠模型 ,从骨代谢学角度探讨双黄益骨方对去卵巢糖尿病大鼠骨质疏松的治疗作用、量效关系及其机理。方法 :将 60只 6月龄雌性清洁级SD大鼠随机分为假手术组、模型组和治疗组 (高剂量中药组 ,低剂量中药组 ,尼尔雌醇组 ) 5组。于成模后及治疗后第 12周末取尿及眼眶静脉窦血液 ,行骨代谢学指标检测 ,并与假手术及模型组对照。结果 :各组体重及雌激素水平均显著低于假手术组 ,HbAlc及BALP则明显增高 (P <0 .0 1) ;另外 ,模型组尿DPD/Cr、Ca/Cr及BGP较假手术组明显增高 ,CT则显著降低 (P <0 .0 5～ 0 .0 1) ;各治疗组尿DPD/Cr、Ca/Cr均显著低于模型组 (P <0 .0 1) ;尼尔雌醇组的雌激素水平显著高于中药组及模型组 (P <0 .0 1) ;中药组CT较模型组、尼尔雌醇组显著升高 (P <0 .0 5 ) ,低剂量中药组差异更显著 (P <0 .0 1) ;各治疗组BGP较假手术组及模型组均有显著升高 ,尤以高剂量中药组升高更显著 (P <0 .0 5 )。结论 :中药和尼尔雌醇能抑制骨吸收、促进骨形成 ,尤以中药组更显著。Objective:To study the preventing and treating, dose-effect relationship, mechanism of Shuanghuangyigu recipe on osteoporosis from the perspective of bone metabolismin postmenopausal osteoporosis diabetes rats induced by ovariectomycombine withstreptozotocininjection.Methods: 150 female SD rats aged 6 monthswere randomly allocated to five group,includingshamoperate group, model group, high-dose decoction treatment group, low-dose decoction treatment group,and nilestriolgroup. Bone metabolism items ofurine and blood comed from orbitvein sinus were evaluated 14 weeks post treatment.Conclusion:Uric DPD/Cr?Ca/Cr in each treatment group were significantly decreased compare with model group(P<0.01);Estrogenlevel was higher in nilestriol group than TCM treatment group or model group(P<0.01); CT increased singnificantly in TCM treatment group compare with model group or nilestriol group(P<0.05),especiallyin low-dose decoction group(P<0.01).BGP showed remarkable increased when each treatment group was compared to shamoperate group or model group,especiallyin high-dose decoction group.Conclusion: Both Shuanghuangyigu Recipe and nilestrion showed beneficialin restraining bone resorptionand promoting bone formation,and Shuanghuangyigu Recipe showed better effect in treating osteoporosis in postmenopausal diabetes rats. Key word: Shuanghuangyigu Recipe;postmenopausal; diabetes mellitus;osteoporosis;animal model; bone metabolism

关 键 词：双黄益骨方 绝经后 糖尿病 骨质疏松 动物模型 骨代谢 骨代谢学 

分 类 号：R274.91[医药卫生—中西医结合] R259.871[医药卫生—中医骨伤科学]