青岛地区2000～2003年肾综合征出血热的病毒基因分型  

ANALYSIS OF THE NUCLEOTIDE SEQUENCE FOR M REGION OF HANTAVIRUS ISOLATED FROM QINGDAO AREA DURING 2000-2003

作　　者：乔刚[1] 张洪乐[1] 逄叔申[1] 龚艳[1] 程茂玲[1] 李震海 钱东萌[3] 王斌[3] 

机构地区：[1]胶南市人民医院,山东胶南266400 [2]青岛市卫生监督所 [3]青岛大学医学院微生物学教研室

出　　处：《齐鲁医学杂志》2004年第2期127-129,共3页Medical Journal of Qilu

摘　　要：①目的　调查山东省青岛地区 2 0 0 0～ 2 0 0 3年汉坦病毒的流行情况 ,研究病毒在该地区的分子流行病学特征。②方法　采集肾综合征出血热 (HFRS)急性期病人血清标本 6 4份 ,提取血清中病毒RNA作为模板 ,根据GenBank中汉坦病毒基因序列 ,设计HTN型和SEO型的通用外套引物 ,同时分别设计HTN型特异性引物和SEO型的特异性引物作为内套引物 ,RT nest PCR扩增汉坦病毒基因组M片段G1区基因序列并测序。测序结果利用DNAStar软件进行核苷酸序列分析。③结果　在 6 4例标本中用HTN型特异性引物扩增阳性 6例 ,占 9% ;用SEO型特异性引物扩增阳性 2 5例 ,占 39% ;总阳性率为 4 8%。总体来看青岛地区流行的汉坦毒株以SEO型为主。SEO型汉坦病毒间核苷酸序列的差异相对较小 ,在核苷酸序列水平其基因的离散度为 0 .3%～ 8.9%。HTN型汉坦病毒的变异率较高 ,其基因的离散度为 2 .6 %～ 11.2 %。④结论　青岛地区汉坦病毒的流行以SEO型为主 ,HTN型并存 。Objective To investigate the prevalence and the molecular epidemiological characteristics of Hantavirus in Qingdao area of Shandong Province during 2000-2003. Methods Serum samples were collected from 64 cases of hemorrhagic fever with renal syndrome(HFRS) and virus RNA was purified. HTN and SEO universal primers were designed as outer primers and HTN and SEO specific primers as inner primers. G1 gene sequence of M fragment from Hantavirus genome was amplied with RT-nest-PCR and analyzed. DNA star software was used to analyze nucleic acid sequence. Results Six(9%)cases were positive with HTN specific primer;25(39%)with SEO specific primer, total positive rate being 48%. SEO type was prevalent in Hantavirus epidemiology in Qingdao area. The variation of the nucleotide sequences among SEO isolates(nucleotide sequence divergence ranging from 0.3%-8.9%) was lower than that among HTN type(nucleotide sequence divergence ranging from 2.6%-11.2%). Conclusion Most isolates of Hantavirus are SEO type, while a few are HTN type in Qingdao. Most HTN isolates were detected at Jiaonan County.

关 键 词：肾综合征出血热 病毒基因分型 汉坦病毒 流行病学 

分 类 号：R512.8[医药卫生—内科学]