分子杂交法检测女性尖锐湿疣中的人乳头瘤病毒DNA  

IDENTIFICATION OF HUMAN PAPILLOMAVIRUSES DNA IN LESIONS FROM WOMEN WITH CONDYLOMA ACUMINATA BY MOLECULAR HYBRIDIZATION

作　　者：沈冰 董小平[1] 黎雪如 房益兰[1] 

机构地区：[1]西安医科大学分子病毒研究室

出　　处：《西安医科大学学报》1992年第3期206-208,T002,共4页Journal of Xi'an Medical University(Chinese)

摘　　要：本研究以^(32)P标记的重组HPV 11,16,18质粒为探针,采用打点杂交法检测了60例女性尖锐湿疣组织(其中外阴47例,阴道12例、宫颈1例)中的HPVDNA;用限制性内切酶和Southern转印杂交法分析了8例HPV 11 DNA的物理状态。结果检出HPV11 19例,其中外阴13例,阴道5例,宫颈1例,阳性率31.7%;HPV16,18各1例,分别检自阴道和外阴,阳性率均为1.7%;HPV 11均以游离状态存在。这一结果为国内进一步深入研究HPV与生殖器癌之间的关系提供了资料。HPV DNA were detected in 60 biopsies from women with condyloma acuminata (vulva 47,Vagina 12,cervix 1)by dot blot hybridiztion;physical states of 8 HPV 11 DNA were analyzed by techniques of restriction endonuclease and Southern blot hybridization. HPV 11 was found in 19biopsies of vulva (13 ). vagina (5)and cervix (1),and HPV 16 and 18 in lvaginal and 1 vulvar biopsy respectively. All of HPV 11 DNA were episomal in cells . Our results provided the data for further studying the relationship between HPV and genital cancer in our country.

关 键 词：人乳头瘤病毒 尖锐湿疣 分子杂交 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]