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机构地区:[1]南京农业大学杂草研究室,江苏南京210095
出 处:《南京农业大学学报》2004年第2期47-50,共4页Journal of Nanjing Agricultural University
基 金:国家 8 63项目 ( 2 0 0 1AA2 460 12 ) ;国家自然科学基金资助项目 ( 3 0 170 619) ;高等学校博士点基金 ( 2 0 0 0 0 3 0 70 8) ;江苏省自然科学基金项目 (BK2 0 0 10 66)
摘 要:通过盆栽试验 ,研究了马唐叶龄、温度、保湿期、保湿期光照时间、孢子接种浓度、喷液量及介质等因子对菌株QZ 2 0 0 0侵染马唐的影响。结果表明 ,三叶一心期以下的马唐幼苗对菌株QZ 2 0 0 0极为敏感 ;病害发生的最适温度范围是15~ 30℃ ;达到理想的致病效果至少需要 2 4h的露水期 ;接种菌液的孢子浓度在 1 0× 10 6mL-1以上 ;在保湿 2 4h的条件下 ,保湿期光照时间越短 ,病害发生越严重 ;油乳剂明显降低菌剂对湿度的需求 ,提高致病力 ;喷液量也显著影响致病效果。结果提示菌株QZ 2 0 0 0可有效控制马唐的危害 ,具有开发为生物除草剂的潜力。The effect of dew duration,inoculum potential,host-plant growth stage,temperature,photoperiod in dew duration,spray volume and media on the pathogenicity of strain QZ-2000 to crabgrass(Digitaria sanguinalis)was studied.It was showed that strain QZ-2000 could cause severe disease on crabgrass seedlings from 1 to 4 leaf stage.For the disease development,the optimal temperature after the inoculation of strain QZ-2000 was 1530 ℃.The pathogenicity of the strain was enhanced with the increase of spore concentration and dew duration.A spore concentration of 10 6 mL -1 caused 100% mortality of the first to third leaf stage plants after being experienced for a 24 h dew duration.The need for a long dew duration(at least 24 h)for infection and disease development could be decreased by adding oil emulsion.Spray volume significantly affected the pathogenic efficiency.It was indicated that the strain QZ-2000 could be used as potential mycoherbicide under specific environmental conditions against crabgrass.
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