不同筋力冬小麦品种子粒中若干淀粉合成关键酶活性变化研究  

Changes in activities of key enzymes of starch synthesis in the kernel of winter wheat cultivars with different gluten strength

作　　者：罗毅[1] 李巧玲 樊国成 王胜强 

机构地区：[1]河南农业大学国家小麦工程技术研究中心,河南郑州450002 [2]许昌市植保植检站,河南许昌461000 [3]新密市种子管理站,河南新密452370 [4]中牟县种子管理站,河南中牟451450

出　　处：《河南农业大学学报》2004年第2期131-134,共4页Journal of Henan Agricultural University

基　　金：河南省重大科技攻关项目(122012300)

摘　　要：在池栽条件下,研究了3个不同筋力(强筋、中筋、弱筋)冬小麦品种子粒灌浆过程中腺苷二磷酸葡萄糖焦磷酸化酶(AGPP)、可溶性淀粉合成酶(SSS)和淀粉分支酶(SBE)3个淀粉合成关键酶活性的变化.结果表明,3个小麦品种的AGPP,SBE和强筋品种的SSS酶活性变化均呈单峰曲线,花后20d达到峰值;中筋和弱筋品种的SSS酶活性则呈双峰曲线,花后10d和20d分别有两个峰值,且第2个峰值显著高于第1个峰值.表明强筋品种子粒中淀粉合成酶底物含量丰富,淀粉合成可能比较活跃,而中筋和弱筋品种子粒中支链淀粉合成可能比较活跃.Changes in activities of three key enzymes AGPP,SSS and SBE associated with starch synthesis were studied during grain filling stage of three winter wheat cultivars with strong,middle and weak gluten force on the condition of pond culture.The results indicated that AGPP and SBE activities of three cultivars and SSS activity of strong gluten cultivars had a single-peak curve in loam soil,their peaks appeared on 20 d after anthesis,but SSS activity of middle and weak gluten cultivars came to be a double-peak curve,the peaks appeared on 10 d and 20 d after anthesis,respectively,and the second peak was much higher than that of the first.It showed that the substrate content of starch synthesis enxymes of strong gluten cultivars in kernel was higher and starch synthesis could be more active than that of middle and weak gluten cultivars,but amylopection synthesis of middle and weak gluten cultivars in kernel could be more active than that of strong gluten cultivars.

关 键 词：面筋 冬小麦 品种 子粒灌浆 淀粉合成酶 酶活性 腺苷二磷酸葡萄糖焦磷酸化酶 可溶性淀粉合成酶 淀粉分支酶 

分 类 号：S512.1[农业科学—作物学]