灰树花DNA对小鼠非特异性免疫功能的影响  

Effects of Grifola frondosa DNA on non-specific immunity of mice

作　　者：阎建忠[1] 吕昌龙[1] 温斌[1] 刘军[1] 曹雅明[1] 吕超[2] 佐佐木甚一[3] 

机构地区：[1]中国医科大学基础医学院免疫学教研室,辽宁沈阳110001 [2]沈阳医学院医疗系 [3]日本弘前大学医学部临床免疫学教研室

出　　处：《中国医科大学学报》2004年第3期203-204,共2页Journal of China Medical University

摘　　要：目的 :以自然杀伤 (NK)细胞杀伤活性、巨噬细胞吞噬功能及TNF α ,IL 1的产生水平为指标 ,研究灰树花DNA对小鼠非特异性免疫功能影响。方法 :用乳酸脱氢酶释放法测定NK细胞杀伤活性 ,吞噬中性红实验法检测巨噬细胞吞噬功能 ,生物活性测定法检测巨噬细胞TNF α和IL 1的产生水平。结果 :灰树花DNA处理组小鼠脾NK细胞杀伤活性 ,巨噬细胞吞噬功能及TNF α,IL 1的产生水平均较生理盐水对照组呈现有显著意义的增高或增强。结论 :灰树花DNA可增强机体非特异性免疫功能 。Objective: To investigate the effects of the DNA extracts of Grifola frondosa on non specific immunity in mouse. Methods: Lactic dehydrogenase assay was used to detect the killing activity of natuural killing (NK) cells. The phagocytosis of macrophages was tested by using phagocytosing neutral red particles. Crystal violet staining and MTT methods were used to detect the levels of TNF α and IL 1produced by the macrophages, respectively. Results: The killing activity of the NK cells, the phagocytosis of macrophages, and the levels of TNF α and IL 1 produced by the macrophages in mice treated with the DNA extracts were all significantly higher than those in the control. Conclusion: Grifola frondosa DNA extracts may promote non specific immunoactivity and inhibit tumor cell growth indirectly.

关 键 词：灰树花DNA 自然杀伤细胞 巨噬细胞 肿瘤坏死因子-Α 白细胞介素-1 

分 类 号：R392.5[医药卫生—免疫学]