烟草花粉母细胞细胞融合过程中细胞骨架的超微结构观察(英文)  

Ultrastructural Observation on the Cellular Skeleton during Cytomixis in Pollen Mother Cells of Tobacco (Nicotiana tobacum)

作　　者：杨军[1,2] 彭正松[1,2] 李卫[3] 高欢欢[3] 郑国锠[3] 

机构地区：[1]西华师范大学珍稀动植物研究所 [2]兰州大学细胞生物学研究所,甘肃兰州730000 [3]兰州大学细胞生物学研究所

出　　处：《云南植物研究》2004年第3期329-337,共9页Acta Botanica Yunnanica

基　　金：国家自然科学基金项目 (39970 70 4 );四川省重点学科建设项目 (SZD0 4 2 0 )资助

摘　　要：应用普通电镜和DGD去包埋技术 ,研究了烟草花粉母细胞中的细胞融合现象及细胞融合过程中细胞骨架的变化。观察发现 ,处于凝线期的花粉母细胞 ,其内含物 ,包括细胞器和染色质 ,主要通过胞质通道向相邻细胞发生转移。DGD去包埋观察发现 ,花粉母细胞中核骨架与细胞质内及胞间连丝和胞质通道内胞质骨架连接成一个整体。在整个细胞融合过程中 ,均有核骨架纤维与染色质相连。Conventional transmission electron microscopy and DGD embedment-free technique were used to investigate cytomixis and the distribution of cellular skeleton filaments in pollen mother cells (PMC) of tobacco ( N.tobacum ).It was observed that cellular contents, including chromatins and organelles,migrated in PMCs through cytoplasmic channels,mainly at the stage of synizesis in meiotic prophase Ⅰ.From DGD embedment-free observation,the nuclear matrix,the cytoskeleton in cytoplasm,as well as in plasmodesmata and cytoplasmic channels,connected the PMCs into an entity.The chromatins were in intimate connection with cellular skeleton filaments,even during the process of migration.The role of cellular skeleton in the process of cytomixis was also discussed.

关 键 词：烟草 花粉母细胞 细胞融合 细胞骨架 超微结构 胞质通道 

分 类 号：Q942[生物学—植物学]