检测CYP2A6基因多态性的PCR技术的基因型方法  

PCR-based genotyping methods for genetic polymorphism of CYP2A6 gene

作　　者：胡春松[1] 朱文玲[2] 程晓曙[1] 苏海[1] 罗伟[1] 洪均言[1] 

机构地区：[1]江西医学院心血管病研究所,江西南昌330006 [2]中国医学科学院北京协和医院心内科,北京100730

出　　处：《中国药理学与毒理学杂志》2004年第3期233-236,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：国家留学基金委员会资助项目 ( 9883 60 0 7)~~

摘　　要：综述检测CYP2A6基因多态性的PCR技术的基因型方法。CYP2A6基因多态性存在于白种人、东方人及非洲裔美国人群中 ,包括CYP2A6 1至CYP2A6 12 ,总共 12个变异等位基因。本文列举了检测CYP2A6基因型的不同的DNA来源、引物和限制性内切酶。已开发PCR技术结合诊断性限制性内切酶分析 (RFLP)如两步PCR和一步PCR RFLP方法检测CYP2A6基因型。此外 ,尚有PCR SSCP +RFLP及直接DNA测序方法。To review PCR based genotyping methods for genetic polymorphism of CYP2A6 gene. Genetic polymorphism of CYP2A6 has been found in Caucasians, Oriental and African American population, which includes from CYP2A6*1 to CYP2A6*12, totally 12 variant alleles. In this article, different DNA source, primers and restriction enzymes for CYP2A6 genotypes were reviewed. Protocols for identification of CYP2A6 genotypes with PCR based techniques combined with diagnostic restriction enzyme digestion such as two step and one step PCR RFLP methods have been developed. Besides, methods of PCR SSCP combined with RFLP and direct DNA sequencing are also introduced.

关 键 词：基因 CYP2A6 基因多态性 PCR 基因型 

分 类 号：R346[医药卫生—基础医学]