碳纳米管原子力显微镜探针对生物样品高分辨率成像的研究  

IMAGING BIOLOGY SAMPLE AT HIGH RESOLUTION WITH CARBON NANOTUBE AFM PROBE

作　　者：国立秋[1] 梁吉[1] 董申[2] 赵铁强[2] 赵清亮[2] 

机构地区：[1]清华大学机械工程系,北京100084 [2]哈尔滨工业大学精密工程研究所,哈尔滨150001

出　　处：《中国生物医学工程学报》2004年第3期243-246,共4页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目 ( 5 0 2 0 2 0 0 6)

摘　　要：本研究将碳纳米管安装到原子力显微镜的标准硅探针上 ,制备了碳纳米管原子力显微镜针尖 ,运用其对生物样品进行高分辨率的成像研究 ,成功地获得了DNA的精细结构和G型免疫球蛋白 (Immunoglobulin G ,IgG)的Y形结构 。The standard probe tips currently employed in AFM have some limitations in biological applications. Carbon nanotubes represent ideal AFM tip materials due to their small diameters, high aspect ratio and unique properties. In this paper carbon nanotubes were attached on the end of standard silicon tips to make carbon nanotube AFM tips. An extremely long lifetime at continuously high resolution of carbon nanotube tip was proved. The nanotube tips were used to image biological samples (DNA and IgG) with tapping mode atomic force microscopy in air. The microstructure of DNA and the characteristic Y-shaped structure of IgG were able to be observed that were previously unobservable with conventional AFM cantilever tips.

关 键 词：碳纳米管 原子力显微镜 针尖 生物分子 

分 类 号：Q336[生物学—遗传学]