旋毛虫肌幼虫核酸疫苗的构建及其在小鼠体内的表达  

Construction of DNA Vaccine of Trichinella spiralis Muscle Larvae and Its Expression in Mice

作　　者：崔晶[1] 张红卫[2] 王中全[2] 李雍龙[1] 

机构地区：[1]华中科技大学同济医学院,武汉430030 [2]郑州大学医学院寄生虫学教研室,郑州450052

出　　处：《中国寄生虫学与寄生虫病杂志》2004年第1期5-8,F003,共5页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：河南省教育厅自然科学研究项目 (2 0 0 2 31 0 0 0 1 4 )~~

摘　　要：目的　构建和表达旋毛虫肌幼虫编码相对分子质量 (Mr) 3 10 0 0抗原结构基因 (TspE1)的重组质粒。　方法　通过逆转录 聚合酶链反应 (RT PCR)特异性扩增TspE1基因 ,构建重组质粒 pUC18 TspE1,并将TspE1基因亚克隆入真核表达载体 pcDNA3中。用基因枪免疫小鼠 ,通过苏木素 伊红 (HE)染色及免疫组化观察重组质粒在皮肤组织内的表达情况。　结果与结论　成功构建 pcDNA3 TspE1重组质粒 。Objective To construct and express recombinant plasmid containing the structural gene encoding {Mr 31 000} antigen of Trichinella spiralis muscle larvae. MethodsThe target gene TspE1 was amplified by RT_PCR, cloned into pUC18 vector, and sub_cloned into the eukaryotic expression vector pcDNA3. BALB/c mice were immunized with the purified recombinant plasmid pcDNA3_TspE1 by gene_gun bombardment. The expression of recombinant plasmid in the skin tissue was observed by HE staining and immunohistochemical staining. Results and Conclusion The recombinant plasmid pcDNA3_TspE1 was successfully constructed and expressed in the BALB/c mice.

关 键 词：旋毛虫 核酸疫苗 抗原 基因枪技术 真核表达 重组质粒 动物实验 

分 类 号：R383.15[医药卫生—医学寄生虫学]