C-myc反义寡脱氧核苷酸抑制类风湿滑膜细胞增生诱导滑膜细胞凋亡  

C-myc antisense oligodeoxynucleotides inhibite synoviocytes proliferation and induce synoviocytes apoptosis

作　　者：赵欣欣[1] 赵丽娟[1] 肖卫国[1] 鲁静[1] 杨聘婷[1] 于清宏[2] 

机构地区：[1]中国医科大学附属一院风湿免疫科 [2]解放军第202医院风湿免疫科

出　　处：《中华风湿病学杂志》2004年第2期67-72,i001,共7页Chinese Journal of Rheumatology

基　　金：辽宁省科技攻关计划资助项目(00225001)

摘　　要：目的研究硫代磷酸化修饰的c-myc反义寡脱氧核苷酸(antisense phosphorothioate oligodeoxynucleotides ,AS P-ODN)在血清存在条件下对培养的人类风湿关节炎(rheumatoid arthritis, RA)滑膜B型细胞增生的影响及诱导凋亡的作用。方法培养RA滑膜B型细胞,合成c-myc AS P-ODN,以阳离子脂质体lipofectin为载体转染滑膜B型细胞,用显微镜观察和四唑盐(MTT)法检测对细胞增生的抑制作用,用荧光染色、荧光显微镜、流式细胞仪检测诱导细胞凋亡和c-myc蛋白表达的变化。结果c-myc AS P-ODN呈序列特异性抑制滑膜B型细胞增生并下调c-myc蛋白表达,诱导滑膜B型细胞凋亡。结论c-myc AS P-ODN可能对RA的滑膜增生性炎症具有潜在的治疗作用,c-myc原癌基因可能成为反义核酸技术治疗RA的靶基因。Objective The c-myc proto-oncogene was over-expressed in synoviocytes from patients with rheumatoid arthritis (RA).This study investigated the effect of c-myc antisense phosphorothioate oligodeoxynucleotides (AS P-ODN) on cultured synoviocytes from patients with RA.Methods AS P-ODN was transfected into cultured human synoviocytes from patients with RA by lipofectin.Inhibition of cell proliferation was assessed by MTT assay and microscopy.Apoptosis was examined by fluorescence staining, fluorescence microscopy and flow cytometry.C-myc protein expression was tested by flow cytometry too.Results C-myc AS P-ODN inhibited synoviocytes proliferation and induced.synoviocytes apoptosis,along with down-regulation of c-myc protein.Conclusion C-myc AS P-ODN may be a useful therapeutic option in treating hyperplasia of the synovial lining cells of RA.

关 键 词：C-MYC 反义寡脱氧核苷酸 滑膜细胞 细胞增生 细胞凋亡 类风湿关节炎 

分 类 号：R593.22[医药卫生—内科学]