曲尼司特对体外培养的人眼小梁细胞转化生长因子-β_2表达的抑制作用  

The inhibitory effect of tranilast on transforming growth factor-β_2 expression in cultured human trabecular meshwork cells

作　　者：曹阳[1] 笪邦红[1] 魏厚仁[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,武汉430022

出　　处：《中华眼科杂志》2004年第4期254-257,共4页Chinese Journal of Ophthalmology

基　　金：国家自然科学基金资助项目 (3 8970 75 8)

摘　　要：目的　观察曲尼司特对体外培养的人眼小梁细胞转化生长因子 β2 (TGF β2 )表达的影响。方法　体外培养的第 3～ 5代人眼小梁细胞经 0 0 (对照组 )、12 5、2 5 0及 5 0 0mg/L曲尼司特处理 4 8h后 ,采用半定量逆转录聚合酶链反应 (RT PCR)观察曲尼司特对体外培养的人眼小梁细胞TGF β2 表达的影响 ,以人甘油醛 3 磷酸酯脱氢酶 (G3PDH)作为内参照。结果　 12 5、2 5 0及 5 0 0mg/L曲尼司特处理组TGF β2 /G3PDH象素值比值分别为 1 85± 0 35 ,1 6 6± 0 4 2 ,1 16± 0 2 4 ,与对照组比值 3 82± 0 5 6比较 ,差异有非常显著意义 (q′ =10 77,11 80 ,14 5 4 ;P <0 0 1) ;同时 ,TGF β2 /G3PDH比值随曲尼司特浓度增加而变小 ,呈现明显的量效关系。结论　曲尼司特能明显抑制小梁细胞TGF β2 的表达。可从发病学途径探讨曲尼司特对原发性开角型青光眼的治疗机制。Objective To investigate the effect of tranilast on transforming growth factor-β_2 (TGF-β_2) expression in cultured human trabecular meshwork cells.Methods TGF-β_2 expression in cultured 3-5 passage human trabecular meshwork cells was measured by semi-quantitative RT-PCR after treated with 0.0 μg/ml(control),12.5,25.0 and 50.0 mg/L tranilast for 48 h. Results The value of TGF-β_2/G3PDH of cells treated with 12.5,25.0 and 50.0 mg/L tranilast was 1.85±0.35,1.66±0.42,1.16±0.24,respectively. The difference between these treated groups and that of the control group (3.82±0.56) was statistically significant ( q ′=10.77,11.80,14.54,P <0.01),respectively. The value of TGF-β_2/G3PDH in the tranilast treated trabecular meshwork cells decreased in a dose-dependent manner. Conclusion Tranilast could inhibit TGF-β_2 expression in cultured human trabecular meshwork cells. It is worth to study the using of tranilast in the treatment of primary open-angle glaucoma. (Chin J Ophthalmol,2004,40:254-257)

关 键 词：曲尼司特 体外培养 小梁细胞 转化生长因子-Β2 抑制作用 原发性开角型青光眼 

分 类 号：R965[医药卫生—药理学]