脊髓NO通路参与APA微囊化牛肾上腺嗜铬细胞蛛网膜下隙移植镇痛机理  

Spinal NO pathway takes part in the analgesic effect of APA microcapsulized BCCs subarachnoid transplantation

作　　者：徐龙河[1] 薛毅珑[2] 罗芸[2] 郭水龙[2] 张宏[1] 

机构地区：[1]解放军总医院麻醉科 [2]解放军总医院老年医学研究所细胞生物学研究室

出　　处：《临床麻醉学杂志》2004年第5期283-285,共3页Journal of Clinical Anesthesiology

基　　金："十五"军队重点课题基金项目 (编号 :Olz0 3 1)

摘　　要：目的　通过观察APA微囊化牛肾上腺嗜铬细胞 (BCCs)移植对坐骨神经慢性挤压伤(CCI)大鼠脊髓神经元型一氧化氮合酶 (nNOS)的影响 ,探讨脊髓NO通路是否参与APA微囊化BCCs蛛网膜下隙移植镇痛机理。方法　SD大鼠分为四组 ,每组 5只 ,即对照组 (C组 ) ,正常大鼠 ;CCI组 ,右侧坐骨神经结扎 ;APA组 ,CCI模型后 1周 ,蛛网膜下隙移植 5 0 0～ 6 0 0个APA空囊 ;APA BCCs组 ,CCI模型后 1周蛛网膜下隙移植 5× 10 6个APA微囊化BCCs。测定各组移植后 1周的感觉异常和痛觉过敏阈值 ,感觉异常以VonFrey细丝触诱发痛阈值 (g)表示 ,痛觉过敏阈值用CO2 激光刺激痛阈值(ms)表示。动物处死后取脊髓冰冻切片 ,按照标准免疫组化法检测各组脊髓中nNOS的表达变化。结果　CCI组、APA组脊髓中nNOS活性较C组明显升高 (P <0 0 1) ,但CCI组和APA组间无显著差异 (P >0 0 5 ) ;APA BCCs组结扎侧触诱发痛阈值和CO2 痛阈值高于CCI组和APA组结扎侧 (P <0 0 1) ,同时脊髓中nNOS活性较CCI组和APA组明显降低 (P <0 0 1) ,与C组基本相同 (P >0 0 5 )。结论　CCI大鼠脊髓nNOS表达增加 ,APA微囊化BCCs蛛网膜下隙移植缓解CCI大鼠的触诱发痛和痛觉过敏 ,同时降低CCI大鼠脊髓nNOS表达 。Objective To study APA microcapsulized bovine chromaffin cells (BCCs) subarachnoid transplantation on the changes of neural nitric oxid synthase(nNOS)in spinal cord of chronic constriction injury (CCI)rats. Methods SD rats were randomly divided into four groups,each has five. Normal rats were used as control group (group C). Rats that right sciatic nerve was ligated were used as CCI group. Five to six hundred empty APA microcapsules(group APA) or 5×10 6 APA microcapsulized BCCs ( group APA-BCCs)were grated into subarachnoid space of CCI rats 7 days after operation. Allodynia and hyperalgesia were measured by Von-Frey filaments and CO 2 laser dividually 7 days after transplantation. Segment four and five of lumbar spinal cord was taken out and 15 μm freezing sections were made 7 days after transplantation.Sections was stained for nNOS according to standard immunocytochemistry. Results The expression of nNOS in the spinal cord of group CCI and group APA was higher than that of group C. In group APA-BCCs, allodynia and hyperalgesia was significantly reduced and the expression of nNOS in the spinal cord was lower than that of group CCI and group APA.There was no difference the expression of nNOS between group BCC and group C. Conclusion The expression of nNOS in the spinal cord of CCI rats was elevated. APA microcapsulized BCCs grafting can alleviate allodynia and hyperalgesia threshold of CCI rats, meanwhile it can decrease the expression of nNOS in the spinal cord of CCI rats. Spinal NO pathway takes part in the analgesic effect of APA microcapsulized BCCs subarachnoid transplantation.

关 键 词：脊髓NO通路 APA微囊化 牛肾上腺嗜铬细胞 蛛网膜下隙移植 镇痛 神经元型一氧化氮合酶 坐骨神经慢性挤压伤 

分 类 号：R614[医药卫生—麻醉学] R651.3[医药卫生—外科学]