扣子七中人参皂苷的HPLC-MS-MS方法研究  

Research of ginsenosides in Kou zi qi using HPLC-MS-MS

作　　者：邹坤[1] 刘朝霞[2] 朱姝[3] 蔡少青[4] 小松かつ子 

机构地区：[1]富山医科药科大学和汉药研究所 [2]三峡大学化学与生命科学学院,湖北宜昌443002 [3]富山医科药科大学和汉药研究所药效解析中心 [4]北京大学药学院生药学研究室,北京100083

出　　处：《药学学报》2004年第5期385-388,共4页Acta Pharmaceutica Sinica

基　　金：湖北省教育厅重大项目(2001Z53008)

摘　　要：扣子七为五加科人参属植物大叶三七 Panaxjaponicus C.A.Mey.var.major(Burkill)C.Y.Wu et K.M.Feng的干燥根茎,分布于云南,重庆,湖北等地[1].在渝东鄂西,扣子七用于养阴,清肺,散淤,止血,定痛[2],其化学成分的研究尚未见报道.Aim To analyze the ginsenosides in Kou zi qi (rhizomes of Panax japonicus C.A. Mey. var. major (Burkill) C. Y.Wu et K. M. Feng), and to supply evidences for chemotaxanology of Panax species and clinical uses of Kou zi qi. Methods The ginsenosides were isolated by HPLC, then the positive- and negative- ion APl-MS/MS of constituents collected from HPLC were measured. Results Eight ginsenosides were identified as ginsenoside Re, ginsenoside Ro, chikuseksusaponins Ⅳ, Ⅳa, notoginsenoside R_2, ginsenosides Rb_1, Rc and Rd, respectively, based on comparison of retention time with those of standards by HPLC, and analysis on their API-MS/MS data. Ginsenoside Ro and chikuseksusaponin Ⅳa are the major components of Kou zi qi. Conclusion This plant had a close relationship to P. stipuleanatus, P. zinginensis and P.japonicus var major; a relatively remote relationship to P. ginseng and P. quinquefolius, in a view of chemotaxanology. Ginsenoside Ro and chikuseksusaponin Ⅳa might be the anti-inflammatory constituents of Kou zi qi.

关 键 词：扣子七 人参皂苷 HPLC-MS-MS 植物药 化学成分 

分 类 号：R282.71[医药卫生—中药学]