免疫细胞化学和DNA图像定量分析在良恶性浆膜腔积液鉴别诊断中的应用  

The Differential Diagnostic Value of Immunocytochemistry andDNA Image Cytometry in Effusions

作　　者：李香菊[1] 潘秦镜[1] 沈桂华[1] 刘秀云[1] 孙耘田[1] 

机构地区：[1]中国协和医科大学中国医学科学院肿瘤医院病理科,北京市100021

出　　处：《中国肿瘤临床》2004年第10期581-583,共3页Chinese Journal of Clinical Oncology

摘　　要：分析B72.3、Ber-EP4和Calretinin抗体组与DNA图像定量分析(DNAimagecytometry,DNA-ICM)结合在良恶性浆膜腔积液诊断和鉴别诊断中的应用价值。方法:选取109例浆膜腔积液标本做石蜡包埋细胞块切片免疫细胞化学(Immunocytochemistry,ICC)和ThinPrep薄片DNA-ICM分析。结果:109例浆膜腔积液中,最终诊断为79例癌性积液和25例良性积液,104例最终诊断病例中,B72.3、Ber-EP4和Calretinin抗体组及DNA-ICM的敏感性分别为88.6%和82.3%,两者结合敏感性高达96.2%。结论:B72.3、Ber-EP4和Calretinin抗体组与DNA-ICM结合能有效提高对癌细胞诊断的敏感性。Objective: To determine the value of the combination of the selected antibody panel and DNA image cytometry (DNA-ICM) in the diagnosis and differential diagnosis of effusions by applying immunocytochemistry (ICC) and DNA-ICM. Methods: ICC applying antibodies B72.3? BerEP4 and Calretinin on paraffin-embedded, formalin-fixed cell block (CB) and DNA-ICM on ThinPrep(TP) slides were performed in the selected 109 effusion specimens. Results: There are 79 carcinomatous effusions and 25 benign effusions in the seleted 109 effusions, the sensitivities of the antibody panel (B72.3?Ber-EP4 and Calretinin) and DNA-ICM for detecting carcimoma cells were 88.6% and 82.3% individually. When they were combined,the sensitivity was 96.2%. Conclusion: Combination ICC applying the antibody panel (B72.3? Ber-EP4 and Calretinin) and DNA-ICM could auxiliarily differentiate metastatic carcinoma cells from reactive mesothelial cells effectively.

关 键 词：浆膜腔积液 癌细抱 免疫细胞化学 DNA图像定量分析 

分 类 号：R446.6[医药卫生—诊断学]