几种趋化性细胞因子及受体基因多态性与系统性红斑狼疮的关联研究  

Study on association between several chemokine polymorphisms and systemic lupus erythematosus

作　　者：叶冬青[1] 胡以松[1] 李向培[2] 杨仕贵[1] 张国庆[1] 

机构地区：[1]安徽医科大学流行病与卫生统计学系教研室 [2]安徽省立医院风湿科

出　　处：《中华风湿病学杂志》2004年第4期196-201,共6页Chinese Journal of Rheumatology

基　　金：国家自然科学基金资助项目(30371247);安徽省自然科学基金项目(98437231);教育部科学技术研究重点资助项目(03056);安徽省教育厅重点科研资助项目(2002kj175ZD)

摘　　要：目的探讨基质衍生因子1-3'A(stromalcell-derivedfactor1-3'A,SDF1-3'A)、趋化性细胞因子受体2-64I(chemoattractantcellreceptor2-64I,CCR2-64I)190位点、单核趋化蛋白1(monocytechemoattractantprotein-1promoter-2518polymorphism,-2518MCP-1)基因多态性及其交互作用与系统性红斑狼疮(SLE)的相关性。方法以143例SLE病人和157名健康对照为对象进行病例对照研究。应用聚合酶链反应—限制性内切酶片段长度多态性(polymerasechainreaction/restrictionfragmentlengthpolymor-phism,PCR-RFLP)方法确定SDF1-3'A和-2518MCP-1基因多态性,应用突变特异性扩增系统(amplifica-tionrefractorymutationsystem,ARMS)确定CCR2-64I基因多态性。结果-2518MCP-1、CCR2-64I、SDF1-3'A等位基因及基因型频率在SLE和健康对照组之间差异无显著性。但-2518MCP-1A/G基因型对照组高于病例组(χ2=4.11,P=0.04)。基因间交互作用分析发现,当SDF1-3'A、-2518MCP-1、CCR2-64I基因型分别为G/G、A/G和G/G时,对SLE发病可能具有保护作用(P<0.05)。其他基因型间均未见交互作用(P>0.05)。结论单个-2518MCP-1、CCR2-64I、SDF1-3'A基因多态性与SLE易感性无关联,但他们之间可能存在交互作用。Objective To investigate the influence of stromal cell-derived factor 1-3'A (SDF1-3'A),chemoattractant cell receptor 2-64I (CCR2-64I),the monocyte chemoattractant protein-1 promoter -2518 polymorphism(-2518MCP-1) and the gene-gene interaction on systemic lupus erythematosus(SLE).Methods The polymorphisms of SDF1-3'A,CCR2-64I and -2518MCP-1 were determined by polymerase chain reaction/restriction fragment length polymorphism(PCR-RFLP) and amplification refractory mutation system (ARMS) respectively.The case-control study was used to explore the association between gene polymorphism and SLE.Results There was no significant difference in distribution of allelic and genotype frequency of SDF1-3'A,CCR2-64I and -2518MCP-1 between SLE and control.But compared with SLE patients,the genotype frequency of A/G of -2518MCP-1 was higher in control.The analysis of gene-gene interaction showed that there was a significant protection effect when the genotype of SDF1-3'A,CCR2-64I and -2518MCP-1 was G/G,A/G and G/G respectively.Conclusion There is no direct effect of SDF1-3'A,CCR2-64I and -2518MCP-1 polymorphism on SLE ,but it is possible that there is gene-gene interaction.

关 键 词：趋化性细胞因子 趋化性细胞因子受体 基因多态性 系统性红斑狼疮 基质衍生因子1 

分 类 号：R593.24[医药卫生—内科学]