血清基质金属蛋白酶-9检测与系统性红斑狼疮活动性的关系  

The relationship between MMP-9 serum level and disease activity in patients with systemic lupus erythematosus

作　　者：刘暘[1] 郑敏[2] 殷文浩[2] 鲍彰[2] 

机构地区：[1]浙江大学医学院附属第二医院风湿病教研室 [2]浙江大学医学院附属第二医院皮肤科

出　　处：《中华风湿病学杂志》2004年第4期220-222,共3页Chinese Journal of Rheumatology

基　　金：浙江省自然科学基金资助项目(399126)

摘　　要：目的探讨血清基质金属蛋白酶-9(MMP-9)与系统性红斑狼疮(SLE)活动程度的关系和意义。方法采用双抗体夹心酶联免疫吸附测定法,检测30名健康人和36例SLE患者血清MMP-9水平,以分析其与SLE活动性变化关系。结果SLE患者血清MMP-9水平[(108±113)ng/ml]明显低于正常对照组[(352±115)ng/ml],P<0.001;糖皮质激素治疗后血清MMP-9水平[(246±196)ng/ml]与治疗前水平[(114±92)ng/ml]相比,差异有显著性意义(P<0.05);SLE患者活动期血清MMP-9水平[(72±66)ng/ml]低于非活动期[(166±146)ng/ml],P<0.05;SLEDAI>8分组[(80±72)ng/ml]低于SLEDAI≤8分组[(152±150)ng/ml],P<0.05;蛋白尿组[(82±20)ng/ml]低于非蛋白尿组[(152±43)ng/ml],P<0.05;关节炎组[(103±126)ng/ml]与非关节炎组[(117±89)ng/ml]之间差异无显著性,P>0.05。结论MMP-9可能与SLE的发病相关,血清MMP-9可作为反映SLE活动程度、肾脏损害及疾病进展或改善的一项指标。Objective To investigate the relationship between serum level of MMP-9(matrix metalloproteinase-9) and the disease activity of systemic lupus erythematosus(SLE).Methods Serum level of MMP-9 of 30 controls and 36 SLE patients were measured by Enzyme Linked Immuno Sorbent Assay.Results Significantly decreased serum level of MMP-9 was found in SLE patients as compared to that in healthy controls(108±113) ng/ml vs (352±155) ng/ml (P<0.001).Serum level of MMP-9 was lower before treatment (114±92)ng/ml than after treatment (246±196)ng/ml (P<0.05).Serum level of MMP-9 was lower in active patients than those with inactive disease,namely(72±66)ng/ml vs (166±146)ng/ml (P<0.05).Serum level of MMP-9 was decreased in patients with proteinuria [(82±20)ng/ml vs (152±43)ng/ml,P<0.05].Serum level of MMP-9 in patients with arthritis [(103±126)ng/ml] and without [(166±146)ng/ml] had no significant difference (P>0.05).Conclusion The present data suggests that MMP-9 may be involved in the pathogenesis of SLE,and serum MMP-9 level may be a marker of disease activity,renal damage,disease progression and amelioration in SLE.

关 键 词：血清 基质金属蛋白酶-9 检测 系统性红斑狼疮 疾病活动性 双抗体夹心酶联免疫吸附 测定 

分 类 号：R593.24[医药卫生—内科学]