黄绿青霉素生物合成及鉴定  

Biosynthesis and evaluation of citreoviridin

作　　者：刘密凤[1] 李德安[1] 李晓丹[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心大骨节病研究所,50001

出　　处：《中国地方病学杂志》2004年第3期255-257,共3页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金资助项目(30271151)

摘　　要：目的生物合成和纯化黄绿青霉素(CIT)。方法市售小米查子加入30%水分,接种黄暗青霉菌孢子后,15～25 ℃培养3周,可获得较高产量的黄绿青霉素。霉变米粉经乙醇提取数次,减压浓缩后,溶解于热苯中,4 ℃过夜并过滤,滴加正己烷于黄色苯溶液中,将生成的黄色沉淀通过硅胶柱,在正己烷∶丙酮(1∶1)中展开蒸干,并溶于甲醇中,形成黄色粉末。结果最终合成的黄绿青霉素成分单一,所含杂质较少。结论实验合成的黄绿青霉素纯度高、杂质少,操作方便、可行性好,便于实验室开展研究工作。Objective To biosynthesize and purify citreoviridin. Methods After inoculation with the P. citreonigrum dierckx spores, the commercial minor ground corn with 30% water added were cultivated at 15～25 ℃ for 3 weeks, a great amount of citreoviridin could be produced. Mouldy corn powder was extracted several times with ethanol, this extract was dissolved in hot benzene after being concentrated, followed by standing overnight at 4 ℃ and filtered,the yellow deposit was precipitated by dropwise addition of n-hexane to the yellow benzene solution. The deposit was dissolved and was added to silica gel column with n-haxane-acetone (1∶1) as solvent, yellow fractionation was evaporated to dryness and dissolved in methanol, yellow powder was obtained. Results The final synthesis citreoviridin contained single component, the impurity was less. Conclusions The synthetic method practiced by this study has good feasibility, is simple and easy, and suitable for laboratory research.

关 键 词：黄绿青霉素 生物合成 鉴定 检测 制备 

分 类 号：TQ466[化学工程—制药化工]