抗Sa、抗KA、抗RA54抗体和RF联合检测对类风湿性关节炎诊断的意义  

Detection and significance of the anti-Sa antibody,anti-keratin,anti-RA54 antibody and rheumatoid factor in patients with rheumatoid arthritis

作　　者：晋淑慧[1] 王艾丽[1] 王娟[1] 江舟[1] 熊华[1] 齐名[1] 武建国[1] 

机构地区：[1]南京军区南京总医院检验科,南京210002

出　　处：《临床检验杂志》2004年第3期178-180,共3页Chinese Journal of Clinical Laboratory Science

摘　　要：目的　探讨抗Sa、抗角蛋白 (抗KA)、抗RA5 4抗体和类风湿因子 (RF)联合检测对类风湿性关节炎 (RA)的诊断意义。方法　用免疫印迹法检测抗Sa、抗RA5 4抗体 ,间接免疫荧光法检测抗KA ,ELISA法检测RF。结果　 12 6例RA患者中 ,抗Sa、抗KA、抗RA5 4和RF的阳性率分别为 4 1.3% ,2 8.6 % ,2 7.8%和 4 6 .0 % ,显著高于 15 9例非RA患者和正常对照者 (P <0 .0 1 。结论　两种以上抗体同时阳性可显著提高诊断RA的特异性 ,有助于早期RA的诊治。Objective To assess and detect the diagnostic significance of anti-Sa antibody、anti-keratin,anti-RA54 antibody and rheumatoid factor in RA patients.Methods Anti-Sa antibody and anti-RA54 antibody were examined by western-blotting.anti-keratin and RF were detected by indirect immunofluorescence and ELISA respectively.Results The positive rates of anti-Sa antibody,anti-keratin,anti-RA54 antibody and rheumatoied factor in 126 patients with RA were 41.3%,28.6%,27.8% and 46%,respectively.The positive rates of antibodies in RA group were higher than those of non-RA groups(P<0.01).Conclusion The positive results of over two antibodies greatly improve the specificity for diagnosis of early RA.

关 键 词：抗SA 抗KA 抗RA54抗体 RF 检测 类风湿性关节炎 诊断 

分 类 号：R593.22[医药卫生—内科学]