冷冻保存同种异体骨-前交叉韧带(ACL)-骨移植实验研究  

An experiment on transplantation of cryopreserved bone anterior cruciate ligament(ACL) bone allograft

作　　者：周建生[1] 张长春[1] 潘功平[1] 胡汝麒[1] 

机构地区：[1]蚌埠医学院附属医院骨科,安徽省蚌埠市233004

出　　处：《中华创伤骨科杂志》2004年第5期546-549,共4页Chinese Journal of Orthopaedic Trauma

基　　金：安徽省自然科学基金资助项目(95-医-16)

摘　　要：目的探讨自体骨-ACL-骨和二步冷冻保存同种异体骨-ACL-骨移植的组织学、形态学及生物力学的变化特点。方法将60只日本大耳兔和60只新西兰兔随机分成自体骨-ACL-骨移植组和二步冷冻保存骨-ACL-骨同种异体移植组。术中及术后不用免疫抑制剂。术后4、8、12周切取移植膝关节及健侧膝关节,行ACL生物力学测试。术后4、12周切取ACL分别作组织学检查和电镜检查。结果自体移植组和二步冷冻保存同种异体骨-ACL-骨移植组的ACL所测最大拉伸强度和拉伸刚度与同期正常对照ACL相比显著下降(P<0.01)两组之间同期相比其最大拉伸强度和拉伸刚度差异无显著(P>0.05)。结论自体骨-ACL-骨移植和二步冷冻保存同种异体骨-ACL-骨移植后具有相同的生物力学性能和组织学愈合过程。Objective To study the histological, morphological and biomechanical characteristics of ACL autograft and two step cryopreserved ACL allograft after transplantation. Methods 60 New Zealand rabbits and 60 Japanese rabbits were randomly divided into two groups: ACL autograft group and two step cryopreserved ACL allograft group. Immunosuppressants were not used after transplantation. Biomechanical and histological examinations and ultramicroscopy of the ACL taken from the transplanted and normal knees were done respectively 4, 8, and 12 weeks after transplantation. Results The maximum tensile strength and stiffness of transplanted ACL were significantly lower in ACL autograft group and two step cryopreserved ACL allograft group than those in the control (P< 0.01). The maximum tensile strength and stiffness of transplanted ACL were not significantly different between ACL autograft group and two step cryopreserved ACL allograft group (P >0.05). Conclusion Two step cryopreserved ACL allograft is similar to ACL autograft in biomechanics and histology.

关 键 词：同种异体移植 生物力学 前交叉韧带 骨移植 冷冻保存 组织学 形态学 

分 类 号：R687.34[医药卫生—骨科学] R318.01[医药卫生—外科学]