油酸-内毒素序贯致伤致老年鼠MODS模型的建立  

Establishment of an MODS model in aged rats

作　　者：徐智[1] 吴国明[1] 钱桂生[1] 王兴胜[1] 陈维中[1] 李淑平[1] 薛桥[2] 王士雯[2] 

机构地区：[1]第三军医大学新桥医院全军呼吸内科研究所 [2]中国人民解放军301医院老年心血管病研究所,北京100853

出　　处：《第三军医大学学报》2004年第10期852-855,共4页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划资助项目 ("973"项目 ) (G2 0 0 0 0 570 0 4 )~~

摘　　要：目的　复制老年鼠多器官功能障碍综合征动物模型 ,为进一步探讨其发病机制奠定基础。方法　将 2 10只2 4月龄Wistar大鼠分为油酸 +脂多糖组 (O +LPS组 )、油酸组 (O组 )、脂多糖组 (LPS组 )。O +LPS组注射油酸 0 175ml/kg ,8h后再注射脂多糖 2 5ml/kg。O组和LPS组分别注射油酸 0 175ml/kg和脂多糖 2 5ml/kg。注射后持续 1 5L/min给氧4h(伤后 1h组除外 )。观测伤前及伤后 1、6、12、2 4、72、12 0h的肺、心、肝、肾、小肠功能变化、全身炎症反应发生率、多器官功能障碍综合征 (MODS)发生率及死亡率。结果　伤后 1～ 2 4h ,O +LPS组的动脉血氧分压 (PaO2 )低于 9 3kPa ,谷丙转氨酶、总胆红素、尿素氮、肌酐、谷草转氨酶、肌酸磷酸激酶、二胺氧化酶的最高值分别达 ( 2 83± 2 61 8)U/L、( 1 65± 0 97)μmol/L、( 18 5± 6 0 )mmol/L、( 76 7± 12 9)mmol/L、( 911 3± 5 16 8)U/L、( 1886± 1876)U/L、( 82 9 3± 3 14 2 10 3 )U/L ,均显著高于伤前自身对照值 (P <0 0 5 ,P <0 0 1)。伤后 6～ 2 4h ,MODS发生率分别为O +LPS组 70 %、LPS组 17 4%、O组15 4%;死亡率分别为O +LPS组 3 3 3 %、LPS组 2 3 3 %、O组 13 3 %。O +LPS组的MODS发生率和死亡率显著高于LPS组和O组 (P <0 0 1)。Objective To establish a rat model of multiple organ dysfunction syndrome (MODS) for the further studies of the pathogenesis of MODS. Methods A total of 210 aged rats (24 month old) were randomly divided into three groups ( n =70): oleic acid plus lipopolysaccharide group (O+LPS), oleic acid group (O), and lipopolysaccharide group (LPS). Rats in O+LPS group received intravenous injection of 0.175 ml/kg (BW) oleic acid at first, and then 2.5 ml/kg (BW) LPS 8 h later. Rats in O group and LPS group, however, received intravenous injection of oleic acid and LPS, respectively. After injection, all rats received 1.5 L/min oxygen therapy for 4 h (except the group at 1 h after injection). The functional changes of the heart, liver, kidney, and small intestine before intravenous injection and at 1, 6, 12, 24, 72, and 120 h after injection were observed. In addition, the incidences of systemic inflammatory response syndrome (SIRS) and MODS, and the mortality of rats in all groups were observed. Results During the period of 1-24 h after injection, the PaO 2 in O+LPS group was less than 9.3 kPa, but the levels of alanine aminotransferase, total bilirubin, urea nitrogen, creatinine, aspartate aminotransferase, creatine phosphokinase, and diamine oxidase were significantly higher than those of the auto control before injection ( P <0.05,<0.01). The mortality of rats in O+LPS group (33.3%) was higher than that in LPS group (23.3%) and in O group (13.3%) ( P <0.01), and the incidence of MODS rats in O+LPS group (70%) was higher than that in rats in LPS group (17.4%) and in O group (15.4%) ( P <0.01). Conclusion The model established by injectionof oleic acid and lipopolysaccharide subsequently, which can successfully imitate the development of MODS after acute lung injury and secondary infection, is a suitable model for the studies of the primordial role of lung in the pathogenesis of the MODS.

关 键 词：老年多器官功能障碍综合征 动物模型 

分 类 号：R-332[医药卫生] R365