PDLLA/HA/DBM复合生物材料对成骨细胞增殖活性的影响  

Effects of PDLLA/HA/DBM biomaterials on the activity of osteoblast proliferation

作　　者：郭乔楠[1] 赵建华[2] 卢佳友[1] 

机构地区：[1]第三军医大学西南医院病理科,重庆400038 [2]第三军医大学大坪医院野战外科研究所骨科,重庆400042

出　　处：《第三军医大学学报》2004年第10期905-907,共3页Journal of Third Military Medical University

基　　金：重庆市攻关项目 ( 2 0 0 3)~~

摘　　要：目的　研究人成骨细胞在一种新型骨替代材料消旋聚乳酸 /羟基磷灰石 /脱钙骨基质 (PDLLA/HA/DBM )上的生长情况 ,并与消旋聚乳酸PDLLA和脱钙骨基质 (DBM)相比较 ,从细胞水平评价材料的生物相容性。方法　将培养的人成骨细胞第 3代 ,密度为 3 5× 10 5/ml悬液 ,分别种植于PDLLA/HA/DBM、PDLLA及DBM上 ,利用细胞计数法检测种植后1、2、3、5、7d的细胞增殖率。结果　成骨细胞在PDLLA/HA/DBM表面的增殖速度较PDLLA和DBM快。Objective To study the effects of PDLLA/HA/DBMV biomaterials on cell proliferation by comparison of polyactic acid (PDLLA) and decalcified bone matrix (DBM) so as to evaluate the biocompatibility of the biomaterials at the cell level. Methods The isolated third generation human fetal osteoblasts (suspension, 3.5×10 5/ml) were inoculated onto the biomaterials of PDLLAHA/DBM, PDLLA, and DBM for 1, 2, 3, 5, and 7 d. Proliferation rates of cells on different materials were determined by flow cytometry. Results The osteoblast proliferation rate on PDLLA/HA/DBM was higher than that on PLA and DBM. Conclusion PDLLA/HA/DBM is compatible for the growth of osteoblasts.

关 键 词：成骨细胞 细胞培养 生物材料 细胞增殖 

分 类 号：R318.08[医药卫生—生物医学工程] R322.71[医药卫生—基础医学]