醋柳黄酮对血管平滑肌细胞胞内游离钙浓度的影响  

Effects of flavonoids of Hippophae rhamnoides L on intracellular free calcium concentration in cultured rabbit aortic smooth muscle cells

作　　者：李家富[1] 章茂顺[2] 王家良[2] 符宗胤[2] 

机构地区：[1]泸州医学院附属医院心内科 [2]华西医科大学附一院心内科

出　　处：《中国临床药理学与治疗学》2004年第3期305-307,共3页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的 :探讨醋柳总黄酮 (TFH)对血管平滑肌细胞胞内游离钙浓度 ([Ca2 +]i)的影响。方法 :采用新一代钙荧光探针Fluo 3 AM检测在高钾、去甲肾上腺素 (NE)、血管紧张素II(AngⅡ )刺激下单层兔主动脉平滑肌细胞内游离钙水平的改变 ,并与传统的钙拮抗剂Verapamil(Ver)进行对照研究。结果 :TFH(10 0mg·L-1)对静息状态的血管平滑肌细胞[Ca2 +]i 无明显影响 ;TFH(6 0～ 10 0mg·L-1)呈剂量依赖性抑制高K+去极化引起的 [Ca2 +]i 升高 ,与Ver作用相似 ,但弱于Ver ;TFH (80、10 0mg·L-1)对NE、AngⅡ通过受体介导引起 [Ca2 +]i 升高均具有明显的抑制作用 ;在无细胞外Ca2 +存在下 ,TFH (80、10 0mg·L-1)对NE引起的 [Ca2 +]i 升高也具有一定程度的抑制效应。结论 :醋柳总黄酮通过对电压依赖性钙通道和受体操纵型钙通道双重抑制降低血管平滑肌细胞内游离钙水平 ,这可能是醋柳黄酮产生舒血管降压作用机制之一。AIM: To explore the effects of total of flavonoid Hippophae rhamnoides L (TFH) on intracellular free calcium ([Ca 2+ ] i) in vascular smooth muscle cells (VSMC). METHODS: The cultured rabbit aortic smooth muscle cells were expose to high K +, norepinephrine (NE) and angiotensin II(Ang Ⅱ), which were compared with those of verapamil(Ver), and then the changes of [Ca 2+ ] i in VSMC were determined by using the new generation of fluorescent Ca 2+ indicater fluo 3/AM. RESULTS: TFH ( 100 mg·L -1 ) had no significant effect on the resting [Ca 2+ ] i. However, andTFH (60- 100 mg·L -1 ) inhibited the elevation of [Ca 2+ ] i induced by high potassium depolarization in a concentration dependent manner. These effects were similar to but weaker than those of Ver. In addition, TFH (80, 100 mg·L -1 ) inhibited the elevation of [Ca 2+ ] i induced by NE or Ang II in the presence of extracellular Ca 2+ . In the absence of extracellular Ca 2+ , TFH (80, 100 mg·L -1 ) also had some blocking effects on the NE induced [Ca 2+ ] i increase. CONCLUSION: TFH may decrease the [Ca 2+ ] i of VSMCs by blocking both voltage dependent calcium channels and receptor operated calcium channels, which may be one of TFH vasodilative and hypotensive mechanisms.

关 键 词：醋柳黄酮 血管平滑肌细胞 胞内游离钙浓度 钙拮抗剂 兔 

分 类 号：R965.1[医药卫生—药理学]