4种归肝经明目中药对晶状体上皮细胞凋亡相关基因Bcl-2和Bax的调控  

Effects of four Chinese herbs which pass through liver-channel on expression of Bcl-2 and Bax in rat lens epithelial cells

作　　者：黄秀榕[1] 祁明信[1] 汪朝阳[1] 王勇[1] 

机构地区：[1]福建中医学院病理生理研究中心

出　　处：《中国临床药理学与治疗学》2004年第3期322-325,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：福建省科技厅重点课题 (№ 98Z173 );福建省卫生厅重点课题(№ 2 0 0 0W60 1)

摘　　要：目的 :探讨 4种归肝经明目中药车前子、青葙子、菊花和熟地对实验性氧化损伤大鼠晶状体上皮细胞 (lensepithelialcell,LEC)凋亡相关基因Bcl 2和Bax的调控。方法 :将SD大鼠双眼随机分成 7组 :空白对照组、过氧化氢 (H2 O2 )组、吡诺克辛 (PS)组、车前子组、青葙子组、菊花组和熟地组。无菌操作摘除眼球并在手术显微镜下分离晶状体 ,使晶状体孵育在 30 0 μmol·L-1H2 O2 培养液中复制LEC凋亡模型 ,同时采用 4种归肝经明目中药干预 ,置二氧化碳培养箱共同孵育 2 4h。取晶状体前囊膜采用免疫组化法检测凋亡相关基因Bcl 2和Bax的蛋白表达并进行比较。结果 :正常SD大鼠LEC中Bcl 2和Bax均有表达 ,Bcl 2表达较Bax表达强 ;H2 O2组Bcl 2表达显著下调 ,Bax表达显著上调 (P <0 .0 1) ,Bcl 2 Bax比率下降 ;与H2 O2 组比较 ,4种归肝经明目中药可明显上调Bcl 2表达 ,下调Bax表达(P <0 .0 1) ,Bcl 2 Bax比率上升 ;PS与 4种归肝经明目中药的调节作用相似 ,但弱于 4种归肝经明目中药的作用 (P <0 .0 5 )。结论 :4种归肝经明目中药调控凋亡相关基因Bcl 2和Bax的表达可能是其抑制LEC凋亡的分子机制。AIM: To investigate the effects of four Chinese herbs which pass through liver channel and improve eyesight,i.e. Semem plantaginis (SP), Semem celosiae (SC), Flos chrysanthemi (FC) and Radix rehmanniae preparata (RRP) on Bcl 2 and Bax,apoptosis related genes,in rat lens epithelial cell (LEC) injured by oxidation. METHODS: Eyes of SD rats were divided randomly into 7 groups: control group, hydrogen peroxide group (H 2O 2), pirenoxine sodium group (PS) and four Chinese herbs (SP, SC, FC and RRP) groups. Eyes were extracted and lenses were separated under operating microscope and sterilized condition. Lenses were incubated in CO 2 incubator for 24 h with 300 μmol·L -1 H 2O 2 and with or without four Chinese herbs. The expression of Bcl 2 and Bax protein of LEC were measured and compared by tearing the LEC anterior capsule via immunohistochemical analysis. RESULTS: Both Bcl 2 and Bax expressed in normal lenses of SD rats, and the expression of Bcl 2 was stronger than Bax. In H 2O 2 group, Bcl 2 expression decreased and Bax expression increased remarkably (P< 0.01 ), and Bcl 2/Bax reduced. In four Chinese herbs groups, there were up regulation in Bcl 2 expression and down regulation in Bax expression, and Bcl 2/Bax increased compared with H 2O 2 group (P< 0.01 ). The changes in PS group were similar to four Chinese herbs groups, but much weaker than those in four Chinese herbs groups (P< 0.05 ). CONCLUSION: Four Chinese herbs regulate the expression of apoptosis related genes,Bcl 2 and Bax,which may be the molecular mechanism of LEC apoptosis inhibition by four Chinese herbs.

关 键 词：中药 车前子 青葙子 菊花 熟地 晶状体上皮细胞 凋亡 基因 

分 类 号：R285.5[医药卫生—中药学] R988.1[医药卫生—中医学]