维持大鼠生理需要最短移植肠管长度的探讨  

The minimal length of intestine graft for normal physical needs of the rats

作　　者：邹小明[1] 李晓林[1] 袁友萍[1] 秦华东[1] 张建国[1] 

机构地区：[1]哈尔滨医科大学附属第二医院普通外科,150086

出　　处：《中华胃肠外科杂志》2004年第3期229-232,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：黑龙江省科学技术计划"攻关"课题(G98G9-25)

摘　　要：目的探讨维持大鼠生理需要最短移植肠管的长度。方法42只Wistar大鼠随机分为肠切除组(切除组):分别切除大鼠小肠远端的80%(切除Ⅰ组)、70%(切除Ⅱ组)和60%(切除Ⅲ组)及盲肠;同系原位小肠移植组(移植组):切除受体全小肠及盲肠,分别移植小肠近端的20%(移植Ⅰ组)、30%(移植Ⅱ组)和40%(移植Ⅲ组);每组6只。观察大鼠术后体重变化,定期采血检测血清总蛋白(Tp)、白蛋白(Alb)、甘油三酯(Tg)和胆固醇(T-ch)的变化,定期做麦芽糖吸收实验;移植组动物死亡时取移植肠管进行苏木精-伊红染色。结果切除Ⅰ组和移植Ⅰ组大鼠分别于术后(14.8±1.3)d和(9.2±1.6)d死于营养不良。切除Ⅱ组4/6只和切除Ⅲ组全部、移植Ⅱ组2/6只和移植Ⅲ组4/6只大鼠存活超过30d。术后30d时,切除Ⅱ组与移植Ⅱ组、切除Ⅲ组与移植Ⅲ组相比,大鼠的体重增长、血清Tp、Alb、Tg、T-ch之间的差异有非常显著性意义(P<0.001)。结论切除大鼠全小肠及盲肠后,至少需要移植30%的近端小肠才能维持其正常的生理需要。Objective To determine the minimal length of intestine graft that can maintain the normal physical needs of rats. Method Forty two Wistar rats were divided into two groups randomly,intestinal resection groups and isogeneic orthotopic intestinal transplantation groups. 80%(groupⅠ), 70%(groupⅡ), 60%(groupⅢ) of distal small bowel and cecum respectively were resected in resection groups. The recipients received 20%(groupⅣ), 30%(groupⅤ), 40%(groupⅥ) of proximal intestine respectively after resecting the total small bowel and cecum in transplantation groups. The body weight, serum total protein(Tp), albumin(Alb), triglyceride(Tg) and cholesterol(T ch) were monitored. Moltose absorption test was performed regularly. Results All the rats in groupⅠand Ⅳdied from malnutrition on (14.8±1.3) and (9.2±1.6)d respectively. 66.7%(4/6) of the rats in groupⅡ, all in groupⅢ, 33.3%(2/6) in groupⅤand 66.7%(4/6) in groupⅥsurvived over 30 days. At the 30th day after the operation, the body weight, Tp, Alb, Tg and T ch values were significantly higher in groupⅡand Ⅴthan those in groupⅢand Ⅵ. Conclusions 30%of proximal intestine is the minimal length of graft to maintain the physical needs after resecting the total small bowel and cecum in rats.

关 键 词：大鼠 小肠移植 肠管长度 手术方法 

分 类 号：R656.7[医药卫生—外科学]