针刺预处理脑组织提取液抗大鼠脑缺血再灌注损伤作用探讨  

Effect of brain tissue extract of rats after acupuncture preconditioning on cerebral ischemia-reperfusion injury

作　　者：陈泽斌[1] 袁芳[1] 梁凤霞[1] 王华[1] 

机构地区：[1]湖北中医学院,武汉430061

出　　处：《中国针灸》2004年第5期347-350,共4页Chinese Acupuncture & Moxibustion

基　　金：湖北省教育厅资助项目 ( 2 0 0 2A0 0 0 0 9)

摘　　要：目的 :证实针刺预处理脑组织提取液的抗脑缺血再灌注损伤作用 ,为针刺效应物质的探讨提供初步的实验依据。方法 :先以“肾俞”“百会”穴针刺预处理大鼠脑组织提取液行大鼠腹腔注射 ,再采用颈动脉引流法脑缺血再灌注造模 ,脑组织石蜡包埋切片 ,HE染色 ,观察脑片缺血性病理变化 ,进行顶皮质Ⅰ区Ⅴ层幸存神经元计数。结果 :在 3个时间段F组幸存神经元密度均显著高于C、D、E 3组 (P <0 0 5 )。结论 :针刺预处理脑组织提取液具有明显的抗脑缺血再灌注损伤作用。Objective To investigate effect of brain tissue extract of rats after acupuncture preconditioning on cerebral ischemia-reperfusion injury. Methods Eighty male wistar rats were randomly divided into six groups: normal control group (group A), sham operation group (group B), model control group (group C), saline group (group D), normal brain tissue extract group (group E) and group of brain tissure extract of rats after acupuncture preconditioning (group F). The cerebral ischemia-reperfusion rat model was made by cervical artery drainage. The rats of the group D, E, and F received respectively intraperitoneal injection of saline, normal brain tissue extract, brain tissue extract from rats needled previously at Shenshu (BL 23) and Baihui (CV 20). The number of the survival neurons in the layer V of the region I in the cerebral parietal cortex was counted after the brain tissue was embedded with paraffin and stained with HE. Results 1 day, 3 days and 7 days after the cerebral ischemia-reperfusion, the survival neuron density of the brain parietal cortex in the group F were higher than those in the group C, D and E (P<0 05). Conclusion The brain tissue extract of rats after acupuncture preconditioning has the effect to resist the cerebral ischemia-reperfusion injury.

关 键 词：再灌注损伤 针灸疗法 脑缺血 针灸效应 预处理 脑组织提取液 大鼠 

分 类 号：R255.2[医药卫生—中医内科学] R246.6[医药卫生—中医学]