滴金法同步检测甲、乙、丙型肝炎病毒免疫标志物方法学建立及评价  

Establishment and Evaluation on DIFA for Synchronous Detection of Immunological Markers of Hepatitis A,B and C Virus

作　　者：王小晋[1] 吴昀[1] 

机构地区：[1]徐州医学院附属淮安医院,江苏淮安223002

出　　处：《现代诊断与治疗》2004年第3期137-139,共3页Modern Diagnosis and Treatment

摘　　要：目的　建立同步检测甲、乙、丙型肝炎病毒免疫标志物的金免疫渗滤法 (GIFA)的实验方法 ,并对其性能作评价。方法　采用胶体金作为免疫示踪物 ,标记羊抗人IgG单克隆抗体 ,将相应的病毒抗原HAV Ag、HBc Ag及HCV Ag包被在硝酸纤维素薄膜表面 ,标本中所含抗体与相应的抗原结合后 ,加入金标记抗体 ,即可在薄膜表面形成肉眼可见的红色斑点。结果　自制的GIFA纸条法检测 64 5份血清标本 ,与ELISA、RIA法作对比 ,符合率分别为 97.4%、96.9%、10 0 .0 1% ,最低检测浓度分别为 2ng/ml、1ng/ml、1ng/ml。 结论　本实验方法具有较高的特异性和灵敏度 ,操作简便。Objective To establish an experimental method of GIFA for synchronous detection of immunological markers of hepatitis A,B and C virus and to evaluate its performance.Method The colloidal golds were used to label monoclonal antibody(SAH-IgG).HAV-Ag,HBc-Ag and HCV-Ag were coated on the surface of pyroxylin membrane.After the antibody in the sample combined with correspond antigen,gold labeled antibodies conjugate were appended ,red spots which could been seen by naked eyes could be formed on the surface of the membrance.Results 645 serum samples were detected by GIFA.Contrasted with those by ELISA and RIA,the corresponding rate was 97.4%,96.9% and 100.0% respectively.The minimumdetective concentration was 2ng/ml,1ng/ml and 1ng/ml repectively.Conclusions DIFA is a specific,sensitive,easy and rapid assay.

关 键 词：检测 甲型肝炎病毒 乙型肝炎病毒 丙型肝炎病毒 免疫标志物 金免疫渗滤法 

分 类 号：R512.6[医药卫生—内科学] R446.6[医药卫生—临床医学]