成肌调节因子MyoD与myogenin在肌肉损伤修复过程的动态变化  

Dynamic changes in the expressions of myogenic regulatory factors MyoD and myogenin during repair of muscle injury

作　　者：曾缨[1] 张成[1] 刘克玄[2] 李才明[1] 冯善伟[1] 李群[1] 柳太云[1] 黄文[1] 

机构地区：[1]中山大学附属第一医院神经内科,广东广州510080 [2]中山大学附属第一医院麻醉科,广东广州510080

出　　处：《第一军医大学学报》2004年第5期542-545,共4页Journal of First Military Medical University

基　　金：国家自然科学基金(30170337);广东省自然科学基金(970061);卫生部临床重点项目(2001321)

摘　　要：目的探讨成肌调节因子MyoD与myogenin在肌肉损伤修复过程中的动态表达。方法用盐酸布比卡因局部注射制作肌肉损伤模型,在损伤后不同时间点取腓肠肌行冰冻切片,HE染色显示损伤肌肉的病理变化,用SABC法检测MyoD与myogenin的表达。结果MyoD在肌肉损伤后18 h开始表达,48 h达高峰;myogenin在肌肉损伤后24 h开始表达,72 h达高峰。结论MyoD与myogenin在肌肉损伤后的再生修复过程中起作用,可作为鉴定肌肉前体细胞和反映肌肉再生的指标。Objective To observe the dynamic changes in the expressions of myogenic regulatory factors MyoD and myogenin during the repair of injured muscle. Methods Muscular injury model was established by local injection of bupivacain, and at different time points following the injection, the gastrocnemius muscles were collected for preparation of cryosections. HE staining was performed for examination of the pathological changes in the injured muscles, and the expressions of MyoD and myogenin were detected by SABC. Results MyoD-positive nuclei began to appear 18 h after muscle injury, reaching the peak till 48 h after the injury. Myogenin-positive nuclei appeared 24 h after muscle injury and peaked at 72 h. Conclusions MyoD and myogenin play a role in muscle regeneration after muscle injury, and they may serve as the indexes to identify muscle precursor cells and mark muscle regeneration process.

关 键 词：成肌调节因子 MYOD MYOGENIN 肌肉损伤 修复过程 动态变化 

分 类 号：R685.4[医药卫生—骨科学]