猪瘟病毒内蒙流行株E2融合基因的构建  

Construction of E2 fusion gene from Neimeng strain of swine fever virus

作　　者：任淑萍[1] 陈越[2] 万敏[1] 包木胜[1] 吴秀丽[1] 于永利[2] 王丽颖[1] 

机构地区：[1]吉林大学基础医学院分子生物学教研室 [2]吉林大学基础医学院免疫学教研室

出　　处：《吉林大学学报（医学版）》2004年第3期329-332,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题 (30 0 70 70 5 )

摘　　要：目的 :获取猪瘟病毒内蒙流行株的 E2基因并构建融合基因。方法 :采用逆转录聚合酶链反应 (RT-PCR)技术从感染猪瘟病毒内蒙流行株猪脾脏中获得并扩增 E2基因特异性片段 ,将扩增的 E2基因测序后与Chaperone1 0相连接构成融合基因并连接到表达载体上。结果 :所获得的猪瘟病毒内蒙流行株 E2基因和其他地区猪瘟病毒流行株 (HCL V株 ) E2基因有 4个碱基的差异 ;融合基因 Chaperone1 0 - E2构建成功。结论 :不同地区猪瘟病毒Objective To obtain the E2 genes from Neimeng strain of classical swine fever virus and construct fusion genes. Methods E2 DNA fragment was acquired from spleen of infected pig and amplified through RT PCR technique. After being sequenced, the E2 gene was ligated to Chaperone 10 to form the fusion gene Chap 10 E2, the fusion gene was ligated to expressing vector. Results There were differences in four bases between E2 gene from Neimeng strain of swine fever virus and E2 gene from HCLV strain. The fusion gene Chap 10 E2 was successfully constructed. Conclusion The difference of E2 gene of swine fever virus from different strains is not significant.

关 键 词：猪瘟病毒 基因 病毒/遗传学 逆转录聚合酶链反应 基因融合 

分 类 号：Q78[生物学—分子生物学]