IFN-PDS合剂对流感病毒感染小鼠T淋巴细胞增殖反应性的影响  

Effects of IFN-PDS on T-lymphocyte proliferation in influenza virus-infected mice

作　　者：曹杰[1] 孙连坤[1] 程晓耕[2] 毛建华[1] 刘喜春[1] 王成[2] 李扬[1] 赵雪俭[1] 

机构地区：[1]吉林大学基础医学院病理生理学教研室,吉林长春130021 [2]长春生物制品研究所,吉林长春130062

出　　处：《吉林大学学报（医学版）》2004年第3期357-359,378,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技发展计划抗非典重点项目 (2 0 0 30 5 0 1 )

摘　　要：目的 :探讨人参二醇皂苷与干扰素 (IFN - PDS)合剂对肺型流感病毒感染小鼠 T淋巴细胞功能的影响。方法 :清洁级昆明系小鼠 6 0只 ,以流感病毒甲 I型鼠肺适应株复制染毒模型。分为对照组 (Con G)、染毒模型组(VIG)、PDS治疗组 (PDSG)、IFN治疗组 (IFNG)及 IFN- PDS治疗组 (IFN- PDSG)。应用 MTT法测定脾和胸腺 T淋巴细胞增殖功能。结果 :1 VIG 脾指数和 Con A 诱导的脾、胸腺细胞增殖反应性明显地高于Con G(P<0 .0 1、P<0 .0 5 ) ;2 PDSG、 IFNG和 IFN- PDSG脾细胞自身增殖反应性 ,均显著地高于 VIG (P<0 .0 1、 P<0 .0 5和 P<0 .0 1 ) ,而 PDSG、IFN- PDSG Con A诱导脾细胞增殖反应性显著低于 VIG(P<0 .0 5 ,P<0 .0 1 ) ;3Con A诱导胸腺细胞增殖反应性 ,PDSG、 IFNG和 IFN- PDSG均低于 VIG (P<0 .0 1、 P<0 .0 5、 P<0 .0 5 )。结论 :流感病毒可直接刺激脾、胸腺 T淋巴细胞增殖 ,并明显地提高 Con A诱导的 T细胞增殖反应性 ;PDS、 IFN - PDS增加流感病毒感染小鼠脾淋巴细胞自身增殖反应性 ,但对 Con A诱导的脾、胸腺细胞过强的增殖反应性有抑制作用 ,显示对 T淋巴细胞功能有双向调节作用。Objective To investigate the effects of interferon panaxadiol saponins (IFN PDS) on the function of T lymphocyte proliferation in influenza virus infected mice. Methods 60 Kunming mice were divided into 5 groups: control group(ConG),influenza virus infected group (VIG),PDS treatment group(PDSG),IFN treatment group (IFNG),IFN PDS treatment group(IFN PDSG). Mouse adopted influenza virus A was used to construct virus infected model. The functions of T lymphocyte proliferation in spleen and thymus gland were observed by MTT assay. Results ①The spleen index of VIG was much greater than that of ConG( P <0 01). The mitogenesis induced by ConA of thymocyte and splenocyte was higher than that in ConG ( P <0 05). ②Self proliferations of splenocytes in PDSG,IFNG, and IFN PDSG were significantly higher than that in VIG( P <0 01, P <0 05, P <0 05). The mitogenesis induced by ConA of splenocytes in PDS and IFN PDSG were lower than that in VIG ( P <0 05, P <0 01). ③The mitogenesis induced by ConA of thymocytes in PDSG,IFNG, and IFN PDSG were lower than that in VIG ( P <0 01, P <0 05, P <0 05),had no difference compared with ConG( P >0 05). Conclusion Influenza virus can stimulate the proliferation of spleen and thymic T lymphocyte directly. It can increase the T lymphocyte mitogenesis induced by ConA obviously. PDS and IFN PDS treatment can increase the self proliferation of splenocyte in influenza virus infected mice, and decrease the excessive mitogenesis of splenocyte and thymocyte induced by ConA. It has two way modulation on the function of T lymphocyte.

关 键 词：人参二醇 人参皂甙 干扰素类 流感病毒A型 T淋巴细胞 

分 类 号：R511[医药卫生—内科学] R-332[医药卫生—临床医学]