金纳多对大鼠全脑缺血再灌注损伤后Fas和Bcl-2蛋白表达的影响  

Effects of Ginaton on expressions of Fas and Bcl-2 protein in rats with cerebral ischemia reperfusion injury

作　　者：韩雪梅[1] 熊文激[2] 胡国中[3] 

机构地区：[1]吉林大学中日联谊医院神经内科,吉林长春130031 [2]吉林大学第一医院干部病房,吉林长春130021 [3]吉林大学中日联谊医院二部预防保健药房,吉林长春130021

出　　处：《吉林大学学报（医学版）》2004年第3期381-383,共3页Journal of Jilin University：Medicine Edition

摘　　要：目的 :研究金纳多对大鼠全脑缺血再灌注损伤的保护作用及对 Fas、 Bcl- 2表达的影响。方法 :建立大鼠全脑缺血再灌注模型 ,应用免疫组织化学方法观察不同再灌注时间脑组织 fas、 bcl- 2的表达 ,以及金纳多给药后的变化。结果 :单纯缺血及再灌注 3h均有 Fas蛋白表达 ,再灌注 6 h Fas蛋白表达达高峰 ,再灌注 2 4 h Fas蛋白表达减少。 Bcl- 2表达于再灌注 3h达高峰 ,再灌注 6 h呈下降趋势 ,2 4 h表达明显减少。金纳多给药组相应时间点 Fas蛋白表达减少 ,Bcl- 2蛋白表达增多。结论 :金纳多对脑缺血再灌注后的细胞凋亡有抑制作用 。Objective To explore the protective effects of Ginaton on cerebral ischemia reperfusion injury and its influence on the exprssions of Fas and Bcl 2 protein in rats. Methods The global cerebral ischemia reperfusion model was established to investigate the expressions of Fas and Bcl 2 protein and the changes after given Ginaton were observed with immunochemistry technique. Results The expression of Fas protein appeared in simple ischemic group and reperfusion 3 hours group, and peaked 6 hours after reperfusion ,and decreased 24 hours after reperfusion. The expression of Bcl 2 peaked 3 hours after reperfusion, and gradually decreased 6 hours after reperfusion, and its expression reduced obviously 24 hours after reperfusion.In Ginaton group, the expression of Fas protein obviously decreased and Bcl 2 protein increased at corresponding time. Conclusion Ginaton can reduce the apoptosis following cerebral ischemia reperfusion and has protective effect on the injury of ischemia and reperfusion.

关 键 词：脑缺血 再灌注 抗原 CD95 原癌基因蛋白质C-BCL-2 金纳多 二裂银杏 

分 类 号：R965[医药卫生—药理学] R-332[医药卫生—药学]