妊娠高血压综合征患者脐血肾上腺髓质素和内皮素的测定及其在脐带血管的表达  

Deternination of adrenomedullin and endothelin in cord blood and their expressions in umbilical cord vessel of patients with pregnancy-induced hypertension

作　　者：阮利红[1] 王鑫[2] 潘宇[3] 朱凤全[1] 

机构地区：[1]吉林大学第二医院妇产科,吉林长春130041 [2]吉林省人民医院心血管内科,吉林长春130021 [3]长春市中心医院妇产科,吉林长春130051

出　　处：《吉林大学学报（医学版）》2004年第3期452-454,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅资助课题 (2 0 0 1 0 4 6 )

摘　　要：目的 :探讨脐带血和脐带血管肾上腺髓质素 (ADM)和内皮素 1 (ET- 1 )在妊娠高血压综合征 (简称妊高征 )中的作用。方法 :应用放射免疫法测定 30例妊高征患者和 1 2例正常妊娠晚期孕妇 (简称正常晚孕组 )脐静脉血浆 ADM和 ET- 1水平 ,用免疫组化法检测 ADM和 ET- 1蛋白在 4 0例妊高征患者和 1 2例正常妊娠晚期孕妇脐带血管中的表达。结果 :1妊高征患者脐静脉血浆 ADM水平高于正常晚孕妇女 ,且随着病情加重逐渐增高 ,中、重度妊高征患者与正常晚孕组比较差异也具有显著性 (P<0 .0 5 )。妊高征患者脐静脉血浆 ET- 1水平略有升高 ,与正常晚孕组比较 ,差异无显著性 (P>0 .0 5 ) ;2正常晚孕组脐带血管内皮细胞和平滑肌细胞有 ADM的表达 ,妊高征组随着病情加重 ,ADM表达增强 ;正常晚孕妇女 ET- 1在脐带血管的内皮细胞表达 ,妊高征组随着病情加重 ,ET- 1表达减弱。结论 :脐血和脐带血管 ADM和 ET-Objective To investigate the role of adrenomedullin (ADM) and endothelin 1 (ET 1) in the pathogenesis of pregnancy induced hypertension (PIH). Methods The plasma concentrations of ADM in human umbilical vein of PIH patients( n =30) and normal late trimester pregnancy women ( n =12)were measured by radioimmunoassay. The expressions of ADM and ET 1 in umbilical cord vessel of PIH patients( n =40)and normal late trimester pregnancy women ( n =12) were detected by immunohistochemistry (SABC) . Results ①The plasma concentration of ADM in human umbilical vein of PIH patients was significantly higher than that of normal late trimester pregnancy women (P<0 05). The plasma concentration of ET 1 in human umbilical vein of PIH patients was similar to that of normal late trimester pregnancy women ( P >0 05). ②The expression of ADM was found in endothelium and smooth muscle cell of umbilical cord vessel,and it increased with the serious degree of PIH. The expression of ET 1 was only found in endothelium of umbilical cord vessel, and it decreased with the serious degree of PIH. Conclusion The changes of ADM and ET 1 in umbilical cord plasma and vessel may related to regulation of fetoplacental circulation in PIH.

关 键 词：妊娠中毒症 EPH 脐血 肽类 内皮缩血管肽1 

分 类 号：R714.24[医药卫生—妇产科学]