微生物来源的抗耐药菌活性化合物SIPI-1140A和SIPI-1140B的研究  

Studies on Compounds SIPI-1140A and B with Inhibitory Activity Against Antibiotic-resistant Microbes from Microorganisms

作　　者：李公克[1] 张琴[1] 江勇[1] 朱宝泉[1] 胡海峰[1] 

机构地区：[1]上海医药工业研究院生物制药部,上海20004

出　　处：《中国天然药物》2004年第3期189-192,共4页

基　　金：上海市自然科学基金资助项目 (No .0 2ZB14 0 94)~~

摘　　要：目的 :分离链霉菌SIPI 114 0菌株生物合成具有抑制耐药菌活性的化合物。方法 :优化发酵条件 ,进行放大发酵 ;发酵液经离心、乙酸乙酯萃取、硅胶柱层析等方法 ,从发酵液中分离出化合物SIPI 114 0A和SIPI 114 0B ,并测定化合物的数据图谱和生物学活性。结果 :通过其理化性质、质谱、紫外、红外和核磁共振等图谱数据的分析 ,确定化合物SIPI 114 0A和B的化学结构 ;生物学活性研究发现两个化合物具有强的抑制甲氧西林耐药的金黄色葡萄菌活性。结论 :链霉菌SIPI 114 0产生的两个活性化合物SIPI 114 0A和B均属于酯肽类抗生素 ,分别与文献报道的QuinomycinA(Echinomycin)和QuinomycinC结构一致。AIM: To isolate compounds from strain SIPI-1140 with inhibitory activity against antibiotic-resistant microbes. METHOD: Compared to fermentation conditions used in the screening,the fermentation technology of strain SIPI-1140 was further improved and the broth was obtained in a large volume;Using ethyl acetate extraction,silica gel column chromatography etc,compound SIPI-1140A and SIPI-1140B were isolated from their broth;the spectra data of the two compounds were determined and their biological activity were studied.RESULT: By the analyses of their physico-chemical properties,MS,UV,IR and NMR spectra data,the structure of SIPI-1140A and SIPI-1140B were identified;the study on biological activity found that two compounds showed strong inhibitory activities against methicillin-resistant Staphylococus aureus.CONCLUSION:The structures of compounds SIPI-1140A and SIPI-1140B produced by strain SIPI-1140 are identical with those of Quinomycin A and Quinomycin C respectively.New inhibitory activities against methicillin-resistant Staphylococus aureus of SIPI-1140A and SIPI-1140B were first reported.

关 键 词：微生物来源 耐药菌活性 化合物 SIPI-1140A SIPI-1140B 链霉菌 醌霉素 抗感染药物 

分 类 号：R978.1[医药卫生—药品]