多丙烯酰胺水凝胶的细胞毒性  

Cytotoxicity of polyarylamide hydrogel

作　　者：方玉 万子义[2] 曹红英 奚廷斐 

机构地区：[1]国家药品监督管理局中检所医疗器械质量监督检验中心,北京100080 [2]北京理工大学材料科学与工程学院,北京100081

出　　处：《大连大学学报》2004年第2期20-21,24,共3页Journal of Dalian University

基　　金：supported by 863 Program(2002AA326040).

摘　　要：本文的目标是要确定从PAM水凝胶提取的丙烯酰胺的容量,并测定提出物的细胞毒性。本文所用的材料是由乌克兰InterfallCo.生产的Gel-Interfall(Lot:030103)。为了测定丙烯酰胺的单体应用了HPLC,并且在单体细胞毒性的计算中包含了MTT的检定。结果表明:在细胞毒性和材料的单体丙烯酰胺容量之间存在着某些关系。The aim of this paper was to determine the acrylamide content in the extracts from PAM hydrogel, and examine the extracts cytotoxicity. The materials used in this paper were Gel-Interfall(Lot: 030103) manufactured by Interfall Co. Ukraine. HPLC was used for detection of acrylamide monomer, and MTT assay was involved in the evaluation of the monomer cytotoxicity. The results indicated that it has some correlation between the cytotoxicity and the monomer acrylamide content of the materials.

关 键 词：PAM 丙烯酰胺 水凝胶 细胞毒性 HPLC 

分 类 号：R313[医药卫生—基础医学] R329.26