野古草种群克隆的遗传变异和遗传结构  

Genetic variability and genetic structure in clonal population of Arundinella hirta

作　　者：洪锐民[1] 王昱生[1] 黄大明[2] 滕小华[1] 中村未树[1] 冈本智伸[3] 

机构地区：[1]东北农业大学动物科学技术学院草地研究室,哈尔滨150030 [2]清华大学生物系生态科学与工程研究所,北京100085 [3]日本国九州东海大学农学部草地生态研究室

出　　处：《生态学报》2004年第5期908-913,共6页Acta Ecologica Sinica

基　　金：国家自然科学基金资助项目 ( 3 0 170 669;3 0 2 70 946) ;国家自然科学基金国际合作研究资助项目 ( 3 0 10 10 3 0 86)~~

摘　　要：用酶电泳法和同工酶分析对东北松嫩草原西北部野古草种群克隆遗传变异性和种群遗传结构做了探讨。讨论了遗传多样性、地理距离和遗传距离之间的关系、大种群和小种群的遗传变异性和种群间的基因流 ;种群间 ,包括大种群和小种群间基因流、遗传和地理距离对遗传多样性的影响、昆虫和风传粉、种群籽苗的补充、遗传多样性的发生和保持 。Authors made approach to genetic variability and genetic structure of 30 clonal populations of Arundinella hirta in northeastern Song Nen steppe in China using enzyme electrophoresis and isozome analysis. Our study obtained the following results: means of P, A, AP, Ho and He in 30 populations were 17.00%, 1.17, 2.03, 0.103 and 0.063, respectively at the population level. The population with highest allelic diversity possessed 38.71% of the total allelic diversity in our sample. All of the alleles encountered in this study can be found in eight populations, including six with unique alleles and two others (RY3 and AS1). These populations appeared in all geographic areas. At the population level, average expected heterozygosity was 0.063. Observed heterozygosity 0.103 and ranging from 0.030 to 0.184. When genetic distances among all population pairs inhabiting the same large population and all pairs inhabiting the small population in the same region were compared, we found no significant relationship between genetic and geographic distances (P>0.56), and no significant difference between the two categories (P>0.89). Genetic diversity, including allelic richness, % polymorphic loci, obeserved heterozygosity, differed signifcantly between large population and small population (P>0.21). In 15 small populations, 57 of the 63 recorded alleles were found, in 15 large populations, 57 of the 63 alleles also were found. Levels of genetic variation did differ, however, among geographic areas within the study area (P>0.04). More than 40% genetic variation (G_(ST)=0.410) may be attributed to the variation among populations. Clonal reproduction and obligate outcrossing of Arundinella hirta complicated identification of other sources of variation. Observed levels of heterozygosity within populations were greater than have been expected if ramets were the products of random sexual reproduction. Values of total inbreedings, which compare observed heterozygosity within population with expected heterozygosity based on random mating

关 键 词：克隆植物 遗传多样性 遗传结构 

分 类 号：Q943[生物学—植物学]