农杆菌介导AFP1基因转化小麦获得转基因植株  

AFP1 gene transformation to wheat by Agrobacterium tumefaciens

作　　者：支大英[1] 徐春晖[1] 薛哲勇[1] 刘庆忠[2] 姜鸿鸣[3] 夏光敏[1] 

机构地区：[1]山东大学生命科学学院,山东济南250100 [2]山东省果树研究所,山东泰安271000 [3]烟台市农业科学研究院,山东烟台265500

出　　处：《山东农业科学》2004年第3期14-16,19,共4页Shandong Agricultural Sciences

基　　金：国家转基因专项J99-0 3 7;山东省重大项目SD990 3资助

摘　　要：以nptⅡ基因为筛选基因,利用农杆菌转化系统向普通小麦品种核生3号和99P的愈伤组织中转入白粉病抗性基因Rs-AFP1;获得抗生素抗性克隆数目分别为27个和24个,其中再生绿苗的克隆分别为2个和8个;PCR分析检测所得nptⅡ阳性植株分别为2株和6株,AFP1基因阳性植株分别为1株和4株。Powdery mildew resistant gene AFP1 was transformed into the calli of wheat (Triticum aestivum L.) cultivar Hesheng3 and 99P by Agrobacterium tumefaciens), and nptⅡ gene was used as selective marker. After cultured on the selective media, paromomycin resistant calli were got at the number of 27 and 24 respectively; 3 and 8 green shoots were regenerated from the paromomycin resistant calli respectively; PCR analysis proved that the (nptⅡ) positive plants were 2 and 6 respectively, and AFP1 positive plants were 1 and 4 respectively.

关 键 词：普通小麦 农杆菌介导转化 转基因植株 Rs-AFP1 

分 类 号：Q78[生物学—分子生物学] S512.11[农业科学—作物学]