日本血吸虫抱雌沟蛋白基因表达的性别与时相差异性研究  

SEX-SPECIFIC AND STAGE DIFFERENT EXPRESSION OF GYNECOPHORAL CANAL PROTEIN GENE OF SCHISTOSOMA JAPONICUM(Chinese strain)

作　　者：金亚美[1] 林矫矫[1] 程国锋[1] 吴祥甫[2] 蔡幼民[1] 

机构地区：[1]中国农业科学院上海家畜寄生虫病研究所 [2]中国科学院上海生物化学研究所,上海200031

出　　处：《寄生虫与医学昆虫学报》2004年第2期70-73,共4页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家高技术"863"基金 (No .2 0 0 1AA2 15 5 1);科技部科研院所社会公益研究专项基金 (No .2 0 0 0 181);国家青年自然科学基金 (No .3 0 10 0 13 8)~~

摘　　要：用Southernblotting ,Northernblotting技术对所克隆的日本血吸虫抱雌沟蛋白编码基因SjGcp进行了性别和时期差异表达研究。结果显示日本血吸虫抱雌沟蛋白基因为雄虫特异性表达基因 ,在感染后第Gynecophoral canal protein shown specific expression in the Gynecophoral canal of male worm and transfer to the female worm.The pairing character of male and female worm was commonly detected after infecting on the 14dyas in the final host.In our research,Southern blotting and Northern blotting were employed to detect the sex-specific and stage-different expression of the SjGCP gene of Schistosoma japonicum.The Southern blotting result shown SjGcp was being in the genome not only of female worm but also of male worm.The Northern blotting results displayed that SjGcp was a male-specific expressing gene,which was detected to be transcripted after infecting of final host when it was just pairing between male and female worm on fourteenth day in the male worm.

关 键 词：日本血吸虫 抱雌沟蛋白基因 性别 时期差异表达 感染 

分 类 号：R383[医药卫生—医学寄生虫学]