氢氟酸烧伤大鼠脂质过氧化损伤与炎症介质变化  

Changes of lipid peroxidation and inflammatory mediators after hydrofluoric acid burn in rats

作　　者：张正孟[1] 阮仕荣[1] 胡安军[1] 盛志勇[2] 王景明[1] 王友臣[1] 

机构地区：[1]中国人民解放军第二五一医院中心实验室,河北张家口075000 [2]中国人民解放军第三○四医院,北京100037

出　　处：《中国烧伤创疡杂志》2004年第2期86-89,共4页The Chinese Journal of Burns Wounds & Surface Ulcers

摘　　要：目的 :为了了解氢氟酸烧伤后 ,机体内部过氧化损伤以及炎性介质变化。方法 :健康大鼠随机分为氢氟酸烧伤组、汽油烧伤A组和B组、正常对照组。氢氟酸烧伤组致 2 %Ⅲ度烧伤。汽油烧伤A组与B组分别致 6 %TBSA及30 %TBSAⅢ度烧伤 ,伤后于 5h、 10h、 2 4h分批活杀。取血清以及肝细胞匀浆检测MDA、NO、TNF α、IL 1β及IL 6等指标。结果 :原始面积为 2 %TBSA的氢氟酸烧伤 ,在 12小时以内迅速扩展至 6 %TBSA。氢氟酸烧伤后 ,MDA、血清NO ,TNF α、IL 1β及IL 6水平显著高于同面积 6 %TBSA普通烧伤动物 ,而与 30 %TBSA普通烧伤组动物接近。 结论 :氢氟酸烧伤后 。Objective: To investigate the changes of lipid peroxidation and inflammatory mediators after hydrofluoric acid burn in rats. Method: Healthy Wistar rats were randomly divided into four groups: hydrofluoric acid burn group, gasoline burn group A, gasoline burn group B, and normal control group, 18 rats in each group. Rats in hydrofluoric acid burn group were burned to 2%TBSA with 3rd degree burn wounds, those in gasoline burn group A were burned to 6%TBSA with 3rd degree, and those in gasoline burn group B to 30%TBSA with 3rd degree burn wounds. The animals were killed respectively at 5h, 10h and 24h post burn and their serum and liver homogenate were examined, the levels of MDA, NO, TNF-α, IL-1 β and IL-1 6 were detected.Result: Rats burned to 2%TBSA by hydrofluoric acid had their burn area expanded to 6%TBSA in 12 hours. The levels of MDA, NO, TNF-α, IL-1 β and IL-1 6 were significantly higher than those burned by gasoline to 6%TBSA and were corresponding to those burned to 30%TBSA by gasoline. Conclusion:Hydrofluoric acid burn resulted in more serious changes in peroxidation injury and inflammatory mediator due to the additive effect of chemical factor and thermal injury.

关 键 词：氢氟酸 烧伤 过氧化损伤 炎性介质 

分 类 号：R643[医药卫生—外科学]