花椒cpDNA trnL-F间隔区序列的特征及其在混淆品鉴别中的应用  

Characteristics of cpDNA trnL-F Sequences of Zanthexylum bungeanum Maxim. and Authentication from Its Forgeries by Those Sequences

作　　者：沈洁[1] 丁小余[1] 张卫明[2] 保曙琳[1] 常俊[1] 唐凤[1] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210097 [2]南京野生植物综合利用研究院,江苏南京210042

出　　处：《中国野生植物资源》2004年第3期29-32,共4页Chinese Wild Plant Resources

基　　金：国家农业领域十五攻关项目 (2 0 0 1BA5 0 2B0 5 )

摘　　要：目的 :通过分析花椒cpDNAtrnL -F间隔区序列特点 ,探讨该序列在花椒与其混淆品鉴别中的意义。方法 :对花椒及其混淆品进行cpDNAtrnL -F间隔区序列比较研究。结果 :花椒cpDNAtrnL -F间隔区序列为 349bp、AT百分比为 6 2 .1%。尽管花椒的果实在外部形态 ,物理特性上与混淆品差异较小 ,但在cpDNAtrnL -F间隔区序列上却存在着显著而稳定的差异。该序列在属以下的分类阶元中同源性极高 ,可达 10 0 %。结论 :根据cpDNAtrnL-F间隔区碱基序列的差异 。Aim:To study the authentication of Zanthexylum bungeanum Maxim. from the fake ones by means of analyzing their cpDNA trnL-F sequences. Methods:compare the cpDNA trnL-F regions between Zanthexylum bungeanum Maxim and fake ones. Results:Length of cpDNA trnL-F of Zanthexylum bungeanum Maxim is 349bp with 62.1percent of AT sites. The difference is obvious between Zanthexylum bungeanum Maxim and fake ones in their cpDNA trnL-F regions though there's little diversity from their appearance of fruits together with physical characters. While this internal space has high alikeness among taxa classified by genus below, reaching full percent parallel. Conclusion:cpDNA trnL-F sequence is only suitable for the authentication of Zanthexylum bungeanum Maxim. from fake ones on the basis of Genus above.

关 键 词：花椒 cpDNAtrnL-F间隔区 序列 基因 果实 植物分类学 芸香科 

分 类 号：Q949.752.7[生物学—植物学]