新Y-STR DYS605在山西汉族人群中的多态性分布  

Study of Polymorphism at New Y-STR DYS605 in a Chinese Han Population of Shanxi

作　　者：张更谦[1] 王艳[1] 张艳霞 徐晓莉[1] 邢小平[1] 王英元[1] 贠克明[1] 

机构地区：[1]山西医科大学法医物证教研室,太原030001 [2]河北省公安厅刑侦局技术处,石家庄05005

出　　处：《遗传》2004年第3期295-297,共3页Hereditas（Beijing)

摘　　要：为了应用更多的Y染色体特异性STR基因座以用于法医学和人类遗传学研究,用PCR结合PAGE技术检测128例山西汉族无关男性DYS605等位基因分布状况。结果显示:山西地区汉族男性DYS605基因座观察到22,21,20,19,18共5个等位基因,基因频率分别为0.0156.0.1797;0.4531;0.2891;0.0625。等位基因20和19之间的电泳距离在非变性胶上非常接近,要有足够的电泳距离才能区分。测序表明该基因座包括3个串联重复区,其中一个为可变重复区。20例女性DNA未发现扩增产物。We study the polymorphism at DYS605, a new tetranucleotide Y-STR locus, in a Chinese Han population of Shanxi to meet the need of more genetic markers in forensic practice and genetic analysis. DNA were extracted from 128 unrelated male venous blood, and amplified using GDB primers. PCR products were detected using non-de- naturing polyacrylamide gel electrophoresis and silver staining. Five alleles named 22, 21, 20, 19, 18 were observed with frequency of 0. 0156; 0. 1797; 0. 4531; 0. 2891; 0. 0625. PCR products were not found in female DNA. Using a long enough gel for a long electrophoresis time is strongly encouraged because the rung between allele 20 and allele 19 is smaller than expected. Allele sequences show that the repetitive units of DY605 were composed with the vari- ant units and non-variant units.

关 键 词：法医学 Y染色体短串联重复序列 遗传多态性 山西 汉族人 DYS605 

分 类 号：Q987[生物学—遗传学]