影响鸡原始生殖细胞分离克隆因素的研究(简报)  

FACTORS INFLUENCING ISOLATION AND CLONING OF CHICKEN PRIMORDIAL GERM CELLS

作　　者：王娟[1] 杜立新[2] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]中国农业科学院畜牧所,北京100094

出　　处：《实验生物学报》2004年第3期247-250,共4页Acta Biologiae Experimentalis Sinica

基　　金：山东省自然科学基金(No.Z99D03)~~

摘　　要：具有多向分化潜能的胚胎干细胞有两种来源:一是来自于早期胚胎内细胞团的胚胎干细胞(Embryonic Stem Cells,ESCs),另一种是来自于胚胎生殖腺原始生殖细胞(Primordial Germ Cells,PGCs)的胚胎生殖细胞(Embryonic Germ Cells,EGCs).Gonadal primordial germ cells (PGCs) were isolated from 4.5-7.5-day-old chickenembryos.Factors influencing isolation and cloning of chicken PGCs were analyzed in this study.The results attained were as follows:Chicken PGCs proliferated fast in presence of 10% FBS.Atprimary culture,PGCs were co-cultured with the gonadal stromal cells.For subculture the chickenPGCs were plated with mitotically inactive feeder layer cells.Further research found that thePMEF (prirnary mice embryonic fibroblast) feeder cells were the most suitable for the growth ofPGCs.When the cell culture medium was supplemented with LIF,FGF,SCF and IGF-Ⅰ,thechicken PGCs were in the best condition.The chicken PGCs were easier to clone when isolatedfrom 5.5-day-old embryonic gonads.In addition,the proper digestion time was 402 when PGCscolonies were passaged.

关 键 词：鸡 原始生殖细胞 分离 克隆 胚胎干细胞 血清浓度 

分 类 号：Q78[生物学—分子生物学]