转基因山羊乳中EPO活性检测  

Detection of Biological Activity of EPO Expressed in Transgenetic Goat Milk

作　　者：汪燕芳[1] 赵建阳 倪亚明[1] 成国祥 

机构地区：[1]同济大学生命科学与技术学院,上海200092 [2]上海转基因研究中心,上海201203

出　　处：《同济大学学报（医学版）》2004年第3期187-188,191,共3页Journal of Tongji University(Medical Science)

摘　　要：目的　对转基因山羊乳中 EPO的生物学活性进行检测。方法　用网织红细胞法检测体内生物活性,用ELISA法和 MTT法检测体外生物活性。结果　羊乳中 EPO体内生物学活性明显低于其体外生物学活性。结论推测我们所得的重组EPO的N糖基高分支的比例小及唾液酸化程度偏低,造成其体内生物活性下降。Objective To detect biological activity of rhEPO expressed in transgenetic goat milk.Methods The biological activity in vivo of rhEPO was determined by reticulacyte counting method.The biological activity in vitro of rhEPO was determined by ELISA and MTT colorimetric method,respectively.Results The biological activity in vivo of rhEPO was obviously lower than its biological activity in vitro.Conclusion The difference is possibly caused by the glycan structure of rhEPO.

关 键 词：红细胞生成素 ELISA法 糖蛋白 

分 类 号：Q819[生物学—生物工程]