唐菖蒲试管培养中根芽形成的研究  

Study on Formation of Radical Buds in Tissue Culture of Gladiola

作　　者：唐蓉[1] 王有庆[1] 王晋民[1] 韦梅琴[1] 

机构地区：[1]青海大学农牧学院农学系,西宁810003

出　　处：《青海农林科技》2004年第2期1-3,共3页Science and Technology of Qinghai Agriculture and Forestry

基　　金：省科技攻关项目部分内容

摘　　要：用组织培养的方法,对唐菖蒲茎尖,茎段进行培养,并研究外植体及生长调节剂对器官形成的影响。结果表明,以茎段作为外植体,愈伤组织分化率可达50%—76 7%;茎尖培养中,在MS培养基中添加6BA1 0mg·l-1和NAA0 1mg·l-1可使愈伤组织分化率达30%左右,培养基中添加BA(或KT)0 5—1mg·l-1并添加生长素类物质NAA0 2—0 5mg·l-1,可显著提高芽分化率,且以6—BA(KT)1mg·l-1添加NAA0 2mg·l-1,对芽形成效果最好,芽诱导率可达100%,芽数分别为7 6和6 8;生根培养中,IBA生根效果优于NAA和IAA,以IBA0 5mg·l-1最佳。The effect on organogenesis formation ,using stem section or stem tip and regulator treatments were studied in gladiola culture.The results showed that when the stem stection of gladiola is used as explants ,the deriving rate of callus were about 50%—76.6%,in stem tip culture ,the deriving rate of callus was 30% when 6—BA1.0mg·l^(-1)and NAA0.1mg·l^(-1) were put in medium, 0.5—2mg·l^(-1) BA or KT were added in the medium,it simulated obviously bud's formation,6—BA1 mg·l^(-1)or KT1mg·l^(-1) was the best concentration.When BA or KT was combined with NAA in the medium,effect of buds formed was the best.Induced rate of bads was 100% The number of buds were 7.6 or 6.8 respectively.In root cultural ,the number of roots was increasing when add to NAA 、IBA IAA in medium,IBA0.5mg·l^(-1)was the best concentration.

关 键 词：唐菖蒲 试管培养 根芽形成 组织培养 外植体 培养基 

分 类 号：S682.24[农业科学—观赏园艺]