益气化瘀方对体外培养的大鼠颈椎间盘软骨细胞超微结构的影响  

Effects of "Qi-Enriching and Blood-Stasis-Resolving Decoction" on Ultrastructure of Chondrocyte in vitro of Cervical Intervertebral Discs in Rats

作　　者：许理忠[1] 侯宝兴[1] 王拥军[1] 施杞[1] 

机构地区：[1]上海中医药大学附属龙华医院,上海,200032

出　　处：《上海中医药大学学报》2004年第2期47-50,共4页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海市科技启明星项目 (0 1QB14 0 39)

摘　　要：研究益气化瘀方 (主药 :黄芪、丹参、川芎等 )对体外培养的大鼠颈椎间盘软骨细胞超微结构的影响。 8～ 10周龄SD大鼠 70只 ,随机均分为生理盐水对照组 ,芬必得组 ,中药低、中、高剂量组 ,每组 14只。定时、定量胃饲相应药物 ,4天后分别处死大鼠取血清 ,相同条件下制备益气化瘀方含药血清和对照血清 ,取体外培养的大鼠颈椎间盘软骨细胞进行研究 ,采用透射电镜及流式细胞仪检测软骨细胞超微结构及细胞凋亡率。结果显示 ,中药组能促进细胞DNA合成(S期 ) ,与对照组相比 ,差异有非常显著的意义 (P <0 0 1)。表明益气化瘀方具有促进大鼠颈椎间盘软骨细胞生长、增殖及促进细胞DNA合成的作用。To study the effects of 'Qi-Enriching and Blood-Stasis-Resolving Decoction' on ultrastructure of chondrocyte,70 SD rats were evenly randomized into five groups and then treated accordingly.Four days later,the serum in each group was collected and added it to the culture in vitro of chondrocyte;the ultrastructure and apoptosis rate of the chondrocyte were observed by transmission electronic microscope and flow cytometer.Results showed that this Decoction could promote the DNA synthesis of chondrocyte,with significant differences compared with other control groups( P <0.01).It is indicated that this Decoction can precipitate the growth,proliferation and DNA synthesis of chondrocyte.

关 键 词：软骨细胞 椎间盘 大鼠 体外培养 益气化瘀方 含药血清 药效学 

分 类 号：R274.981.55[医药卫生—中西医结合]