BNYVV CP基因的克隆及载体构建  

Cloning of cDNA for BNYVV CP Gene and Construction of Transforming Vector

作　　者：斯钦巴特尔[1] 莫日根[2] 哈斯阿古拉[2] 张剑峰 

机构地区：[1]内蒙古农业科学院,内蒙古呼和浩特010031 [2]内蒙古大学,内蒙古呼和浩特010000 [3]内蒙古甜菜制糖工业研究所,内蒙古呼和浩特010000

出　　处：《华北农学报》2004年第2期37-39,共3页Acta Agriculturae Boreali-Sinica

摘　　要：以甜菜丛根病根或病叶总RNA为模板,经反转录PCR扩增合成编码BNYVV CP的全长cDNA,将其克隆于pUCm-T载体上,获得了重组质粒pUCm-T/CP。经序列分析,此cDNA为一个567 bp长的开放阅读框架,编码由188个氨基酸组成的病毒外壳蛋白。又将CP基因构建到植物双元载体pROKⅡ中。最后通过三亲融合,得到了融合农杆菌LB4404。Identification and characterization of a full-length cDNA encoding for BNYVV coat protein was described. The cDNA was 567 bp in length with open reading frame of 188 amino acids. The CP gene was inserted into the plant binary vector pROKII. And finally the recombinant vector pROKII was infused into Agrobac-terium tumefactions.

关 键 词：甜菜 丛根病 BNYVV CP基因 基因克隆 转化载体 抗病基因 转基因 

分 类 号：S435.663[农业科学—农业昆虫与害虫防治] S566.3[农业科学—植物保护]